This second edition of Protein NMR Techniques is well written with a dynamic approach that covers multiple topics within its nineteen chapters. The text opens with a review of recombinant protein expression using two organisms, E. coll and P. pastoris that can produce high yields of isotopically labeled protein at a reasonable cost. The focus then shifts slightly to studies of aligned molecules, starting with a chapter on different options for the preparation of an aligned sample. The text also provides a comprehensive review of the use of RDCs to the study protein dynamics highlights and the range of information accessible using these methods. The book concludes with a concise explanation of the application of solid-state methods and the study of membrane proteins, a particularly important but difficult class of targets. Protein NMR Techniques, Second Edition will prove an invaluable resource to biochemists and researchers alike.

Table of Contents:
1 Screening and optimizing protein production in E. coli Lorraine Hewitt and James M. McDonnell; 2 Isotopic labelling of recombinant proteins from the methylotrophic yeast Pichia pastoris Andrew R. Pickford and Joanne M. O Leary; 3 Perdeuteration/site-specific protonation approaches for high MW proteins Stephen Matthews; 4 Segmental isotopic labelling for structural biological applications of NMR David Cowburn, Alexander Shekhtman, Rong Xu, Jennifer J. Ottesen, and Tom W. Muir; 5 TROSY-based correlation and NOE spectroscopy for NMR structural studies of large proteins Guang Zhu, Youlin Xia, Donghai Lin, Kong Hung Sze, and Xiaolian Gao; 6 Media for studies of partially aligned states Kieran Fleming and Stephen Matthews; 7 Residual dipolar couplings in protein structure determination Eva de Alba and Nico Tjandra; 8 Projection angle restraints for studying structure and dynamics of biomolecules Christian Griesinger, Wolfgang Peti, Jens Meiler, and Raphael Br schweiler; 9 Characterizing domain interfaces by NMR Luke M. Rooney, Sachchidanand, and Jrn M. Werner; 10 Characterization of the overall rotational diffusion of a protein from 15N relaxation measurements and hydrodynamic calculations Jennifer Blake Hall, Oliver Walker, and David Fushman; 11 TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding Guang Zhu, Youlin Xia, Donghai Lin, Kong Hung Sze, and Xiaolian Gao; 12 Measurement of intermediate exchange phenomena James G. Kempf and J. Patrick Loria; 13 NMR studies of partially-folded molten globule states Christina Redfield; 14 Structure determination of protein complexes by NMR Daniel Nietlispach, Helen R. Mott, Katherine M. Stott, Peter R. Nielsen, Abarna Thiru and Ernest D. Laue; 15 NMR studies of protein - nucleic acid interactions Gabriele Varani, Yu Chen, and Thomas C. Leeper; 16 Using NMR View to visualize and analyze the NMR spectra of macromolecules Bruce A. Johnson; 17 Automated NMR structure calculation with CYANA Peter Gntert; 18 NOE assignment with ARIA 2.0: the nuts and bolts Michael Habeck, Wolfgang Rieping, Jens P. Linge, and Michael Nilges; 19 Membrane protein structure determinations using solid state NMR Anthony Watts, Suzana K. Straus, Stephan L. Grage, Miya Kamihira, Yuen Han Lam, and Xin Zhao