2D and 3D Cellular Screening Models and AI Guided Analysis, Volume 204 explores the development, application, and comparative benefits of 2D monolayer cultures and 3D organoids, spheroids, and tissue-engineered constructs for biomedical research and drug discovery. Chapters in this new release include High throughput CellTiter-glo viability assessment, Assessing viability of cancer cell spheroids in 3D drug screening, 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets, Use of micropatterning for 3D cell culture, Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture models, and much more. Additional chapters cover A 3D model for assessing regulated cell death modalities, YOLO detection model for Cell Viability assessment, Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care, Automated image analysis and machine learning for the assessment of mitochondrial membrane potential, Assessing cancer cell heterogeneity in patient-derived tumoroids, Assessment of lysosomal drug sequestration and release using fluorescence microscopy, Assessment of T cell toxicity in ex-vivo models of cancer, Three-dimensional Organoid Screening using Leica Thunder Microscope, Using vascularized organoids-on-chip for assessing systemic drug delivery, and many more interesting and timely topics.

Table of Contents:
1. Preface Guido Kroemer and Oliver Kepp 2. High throughput CellTiter-glo viability assessment Dana Alawieh and Aline Renneville 3. Assessing viability of cancer cell spheroids in 3D drug screening Oliver Kepp and Marion Leduc 4. 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets Charles Baroud 5. Use of micropatterning for 3D cell culture Emna Ouni and Kristine Schauer 6. Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture models Philip Wong, Rodin Chermat, Elena Refet-Mollof, Maryam Ziaee, Julie Lafontaine and Thomas Gervais 7. A 3D model for assessing regulated cell death modalities Dmitri Krysko and Robin Demuynck 8. YOLO detection model for Cell Viability assessment Felix Peyre 9. Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care Zeeshan Ahmed, Kritika Singh, Sarah Usman, Saman Zeeshan, Naveena Yanamala, Vikram Bhise, Mark Nichols, Sabahat Bokhari and Partho Sengupta 10. Automated image analysis and machine learning for the assessment of mitochondrial membrane potential Marta Giacomello, Camilla Bean, Federica Dal bello, Emad Norouzi Esfahani, Alessio Gianelle, Saradha Ramesh, Luca Persano, Elena Rampazzo and Caterina Vianello 11. Assessing cancer cell heterogeneity in patient-derived tumoroids Flora Doffe 12. Assessment of lysosomal drug sequestration and release using fluorescence microscopy Karla Alvarez, Mojgan Djavaheri-Mergny, Guido Kroemer, Lucille Ferret, Samy Dehissi and Jean Guillon 13. Assessment of T cell toxicity in ex-vivo models of cancer Emmanuel intégrative des tumeurs et immunothérapie Donnadieu and Alice Machado 14. Three-dimensional Organoid Screening using Leica Thunder Microscope Manoliu Tudor 15. Using vascularized organoids-on-chip for assessing systemic drug delivery Xavier Gidrol, Emily Tubbs, Fabrice P Navarro and Yves Fouillet 16. Quantitative assessment of nanoparticle uptake and trafficking in 3D cell culture models Meritxell B. Cutrona 17. Fluorescence microscopic quantitation of lysosomal cholesterol accumulation induced by cationic amphiphilic drugs Mojgan Djavaheri-Mergny, Karla Alvarez, Samy Dehissi, Lucille Ferret and Guido Kroemer