Bioconjugate Techniques

Bioconjugate Techniques

          
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About the Book

Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

Table of Contents:
PART I Bioconjugate Chemistry 1. Functional Targets 2. The Chemistry of Reactive Groups PART II Bioconjugate Reagents 3. Zero-Length Cross-Linkers 4. Homobifunctional Cross-linkers 5. Heterobifunctional Cross-linkers 6. Trifunctional Cross-linkers 7. Dendrimers and Dendrons 8. Cleavable Reagent Systems 9. Fluorescent Probes 10. Bifunctional Chelating Agents and Radioimmunoconjugates 11. Biotinylation Reagents 12. Iodination Reagents 13. Silane Coupling Agents 14. Microparticles and Nanoparticles 15. Buckyballs, Fullerenes, and Carbon Nanotubes 16. Mass Tags and Isotope Tags 17. Chemoselective Ligation; Bioorthogonal Reagents 18. Discrete PEG Compounds PART III Bioconjugate Applications 19. Preparation of Hapten - Carrier Immunogen Conjugates 20. Antibody Modification and Conjugation 21. Immunotoxin Conjugation Techniques 22. Preparation of Liposome Conjugates and Derivatives 23. Avidin - Biotin Systems 24. Preparation of Colloidal-Gold-Labeled Proteins 25. Modification with Synthetic Polymers 26. Enzyme Modification and Conjugation 27. Nucleic Acid and Oligonucleotide Modification and Conjugation 28. Bioconjugation for the Study of Protein Interactions


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Product Details
  • ISBN-13: 9780123705013
  • Publisher: Elsevier Science Publishing Co Inc
  • Publisher Imprint: Academic Press Inc
  • Depth: 51
  • Height: 235 mm
  • No of Pages: 1232
  • Spine Width: 44 mm
  • Width: 191 mm
  • ISBN-10: 0123705010
  • Publisher Date: 03 Jun 2008
  • Binding: Hardback
  • Edition: 2
  • Language: English
  • Series Title: English
  • Weight: 1810 gr


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