Expert laboratorians update the classic bioconjugation methods and introduce valuable new techniques that go beyond pure biomedical applications to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic force microscopy.

Table of Contents:
Part I. Antibody and Enzyme Conjugates Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies: Nanoconjugates for Protection Against Endothelial Oxidative Stress Vladimir V. Shuvaev, Thomas Dziubla, Rainer Wiewrodt, and Vladimir R. Muzykantov Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals Silvia Muro, Vladimir R. Muzykantov, and Juan-Carlos Murciano Smart Polymer-Streptavidin Conjugates Patrick S. Stayton, Zhongli Ding, and Allan S. Hoffman Conjugates of Peptides and Proteins to Polyethylene Glycols Margherita Morpurgo and Francesco M. Veronese Chemical Production of Bispecific Antibodies Robert F. Graziano and Paul Guptill Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties Carl-Wilhelm Vogel Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase Markus Meusel Part II. Nucleic Acid Conjugates Fluorescent Sample Labeling for DNA Microarray Analyses Verena Beier, Andrea Bauer, Michael Baum, and Jorg D. Hoheisel High-Density Labeling of DNA for Single Molecule Sequencing Susanne Brakmann Sequence-Specific DNA Labeling Using Methyltransferases Goran Pljevaljcic, Falk Schmidt, Alexander Peschlow, and Elmar Weinhold Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications Michael Adler Covalent Coupling of DNA Oligonucleotides and Streptavidin Florian Kukolka, Marina Lovrinovic, Ron Wacker, and Christof M. Niemeyer Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates David R. Corey Synthesis of Peptide Nucleic Acid-Peptide Conjugates Kunihiro Kaihatsu and David R. Corey Part III. Glycosyl and Lipid Conjugates Protein Lipidation Jurgen Kuhlmann Synthesis of Lipidated Peptides Ines Heinemann, Martin Volkert, and Herbert Waldmann In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins Thomas Durek, Roger S. Goody, and Kirill Alexandrov Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling Melanie Wagner and Jurgen Kuhlmann Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments: Semisynthesis of Glycosylated Interleukin-2 Thomas J. Tolbert and Chi-Huey Wong Subtilisin-Catalyzed Glycopeptide Condensation Thomas J. Tolbert and Chi-Huey Wong Part IV. Biofunctionalization of Surfaces Peptide Nucleic Acid Microarrays Anette Jacob, Ole Brandt, Achim Stephan, and Jorg D. Hoheisel Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles Pompi Hazarika, Tatiana Giorgi, Martina Reibner, Buelent Ceyhan, and Christof M. Niemeyer Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging Adam T. Woolley Index