The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Table of Contents:
Transport Theory:G. Sachs and S. Fleischer, Transport Machinery: An Overview.M. Klingenberg, Survey of Carrier Methodology: Strategy for Identification, Isolation, and Characterization of Transport Systems.W.D. Stein, Kinetics of Transport: Analyzing, Testing, and Characterizing Models Using Kinetic Approaches.O.S. Andersen, Kinetics of Ion Movement Mediated by Carriers and Channels.R. Dev~aaes and R.M. Krupka, Inhibition Kinetics of Carrier Systems.J.V. Vadgama, Design of Simple Devices to Measure Solute Fluxes and Binding in Monolayer Cell Cultures.W.P. Jencks, Utilization of Binding Energy and Coupling Rules for Active Transport and Other Coupled Vectorial Processes.K.R. Runyan and R.B. Gunn, Generation of Steady-State Rate Equations for Enzyme and Carrier-Transport Mechanisms: A Microcomputer Program.Model Membranes and Their Characteristics:M.C. Woodle and D. Papahadjopoulos, Liposome Preparation and Size Characterization.T. Kondo, Preparation of Microcapsules from Human Erythrocytes: Use in Transport Experiments of Glutathione and Its S-Conjugate.H. Schindler, Planar Lipid-Protein Membranes: Strategies of Formation and of Detecting Dependencies of Ion Transport Functions on Membrane Conditions.D. Zakim and A.W. Scotto, Spontaneous Insertion of Integral Membrane Proteins into Preformed Unilamellar Vesicles.N.A. Dencher, Gentle and Fast Transmembrane Reconstitution of Membrane Proteins.R. Benz, H.-A. Kolb, P. L~adauger, and G. Stark, Ion Carriers in Planar Bilayers: Relaxation Techniques and Noise Analysis.D.W. Urry, T.L. Trapane, C.M. Venkatachalam, and R.B. McMichens, Ion Interactions at Membranous Polypeptide Sites Using Nuclear Magnetic Resonance: Determining Rate and Binding Constants and Site Locations.D. Cafiso, A. McLaughlin, S. McLaughlin, and A. Winiski, Measuring Electrostatic Potentials Adjacent to Membranes.H. Rottenberg, Determination of Surface Potential of Biological Membranes.P. Fromherz, Lipid Coumarin Dye as Probe of Interfacial Electrical Potential in Biomembranes.R. Kr~mer, Modulation of Membrane Protein Function by Surface Potential.Isolation of Cells:D. Pietrobon and S.R. Caplan, Use of Nonequilibrium Thermodynamics in the Analysis of Transport: General Flow-Force Relationships and the Linear Domain.M.J.M. Lewin and A.M. Cheret, Cell Isolation Techniques: Use of Enzymes and Chelators.T.G. Pretlow and T.P. Pretlow, Cell Separation by Gradient Centrifugation Methods.M.J. Sanders and A.H. Soll, Cell Separation by Elutriation: Major and Minor Cell Types from Complex Tissues.J. Wells, Cell Separation Using Velocity Sedimentation at Unit Gravity and Buoyant Density Centrifugation.H-G. Heidrich and K. Hannig, Separation of Cell Populations by Free-Flow Electrophoresis.P-A. Albertsson, Separation of Cells and Cell Organelles by Partition in Aqueous Polymer Two-Phase Systems.B.D. Jensen and P.K. Horan, Flow Cytometry: Rapid Isolation and Analysis of Single Cells.A. Garcia-Perez, W.S. Spielman, W.K. Sonnenburg, and W.L. Smith, Use of Cell-Specific Monoclonal Antibodies to Isolate Renal Epithelia.J.D. Gardner and R.T. Jensen, Pancreatic Acini as Second Messenger Models in Exocrine Secretion.R.M. Johnstone and P.C. Laris, Ascites Cell Preparation: Strains, Caveats.Polar Cell Systems:W.S. Rehm, M. Schwartz, and G. Carrasquer, Direct Current Electrical Measurement in Epithelia: Steady-State and Transient Analysis.C. Clausen, Impedance Analysis in Tight Epithelia.L.G.M. Gordon, G. Kottra, and E. Fr~adomter, Electrical Impedance Analysis of Leaky Epithelia: Theory, Techniques, and Leak Artifact Problems.O.H. Petersen, Patch-Clamp Experiments in Epithelia: Activation by Hormones or Neurotransmitters.P.H. Barry, Ionic Permeation Mechanisms in Epithelia: Biionic Potentials, Dilution Potentials, Conductances, and Streaming Potentials.S.A. Lewis and N.K. Wills, Use of Ionophores in Epithelia: Characterizing Membrane Properties.J.S. Handler, N. Green, and R.E. Steele, Cultures as Epithelial Models: Porous-Bottom Culture Dishes for Studying Transport and Differentiation.A.D.C. Macknight and J.P. Leader, Volume Regulation in Epithelia: Experimental Approaches.J.K. Foskett and C. Scheffey, Scanning Electrode Localization of Transport Pathways in Epithelial Tissues.Modification of Cells:P.F. Baker and D.E. Knight, Experimental Control of Intracellular Environment.A. Loyter, N. Chejanovsky, and V. Citovsky, Implantation of Isolated Carriers and Receptors into Living Cells by Sendai Virus Envelope-Mediated Fusion.P.S. Uster and R.E. Pagano, Resonance Energy Transfer Microscopy: Visual Colocalization of Fluorescent Lipid Probes in Liposomes.G.A. Weisman, K.D. Lustig, I. Friedberg, and L.A. Heppel, Permeabilizing Mammalian Cells to Macromolecules.Author Index.Subject Index.