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Biothermodynamics, Part C: Volume 488(Volume 488 Methods in Enzymology)

Biothermodynamics, Part C: Volume 488(Volume 488 Methods in Enzymology)

          
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About the Book

In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. Biothermodynamics, Part C presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an “energy book-keeping system.? While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.

Table of Contents:
Measurement and Analysis of Equilibrium Binding Titrations: A beginner’s guide Dorothy Beckett Macromolecular Competition Titration Method: Accessing Thermodynamics of the Unmodified Macromolecule – Ligand Interactions Through Spectroscopic Titrations of Fluorescent Analogs Wlodzimierz Bujalowski and Maria J. Jezewska Analysis of PKR-RNA interactions by sedimentation velocity C. Jason Wong, Katherine Launer-Felty and James L. Cole Structural and Thermodynamic Analysis of PDZ/Ligand Interactions Tyson R. Shepherd and Ernesto J. Fuentes Thermodynamic analysis of metal ion-induced protein assembly Andrew B. Herr and Deborah G. Conrady Thermodynamic Dissection of Colicin Interactions Nicholas G Housden and Colin Kleanthous Energetics of Src homology domain interactions in receptor tyrosine kinase-mediated signaling John E. Ladbury and Stefan T. Arold Structural and Functional Energetic Linkages in Allosteric Regulation of Muscle Pyruvate Kinase J. Ching Lee and Petr Herman Analysis of Free Energy versus Temperature Curves in Protein Folding and Macromolecular Interactions Vince J. LiCata and Chin-Chi Liu Application of the Sequential n-Step Kinetic Mechanism to Polypeptide Translocases Aaron L. Lucius, Justin M. Miller and Burki Rajendar A Coupled Equilibrium Approach to Study Nucleosome Thermodynamics Andrew J. Andrews and Karolin Luger Quantitative methods for measuring DNA flexibility in vitro and in vivo Justin P. Peters, Nicole Becker, Emily M. Rueter, Zeljko Bajzer, Jason D. Kahna and L. James Maher A Thermodynamic Approach for the Targeting of Nucleic Acid Structures Using Their Complementary Single Strands Hui-Ting Lee, Caroline Carr, Hollie Siebler, Lela Waters, Irine Khutsishvili, Fany Iseka, Brian Domack, Chris M. Olsen, and Luis Marky Thermodynamics of Biological Processes Hernan G. Garcia, Jane Kondev, Nigel Orme, Julie Theriot and Rob Phillips Protein stability in the presence of co-solutes Luis Marcelo F. Holthauzen, Matthew Auton, Mikhail Sineva and Jorg Rosgen Small Angle X-ray Scattering Studies of Peptide-Lipid Interactions using the Mouse Paneth Cell α-Defensin Cryptdin-4 Abhijit Mishra, Kenneth P. Tai, Nathan W. Schmidt, André J. Ouellette and Gerard C. L. Wong Synergy of molecular dynamics and isothermal titration calorimetry in studies of allostery Rebecca Strawn, Thomas Stockner, Milan Melichercik, Lihua Jin, Wei-Feng Xue, Jannette Carey and Rüdiger Ettrich Using tryptophan fluorescence to measure the stability of membrane proteins folded in liposomes C. Preston Moon1 and Karen G. Fleming Non-B Conformations of CAG Repeats Using 2-aminopurine Natalya N. Degtyareva and Jeffrey T. Petty Disulfide bond-mediated passenger domain stalling as a structural probe of autotransporter outer membrane secretion in vivo Jonathan P. Renn & Patricia L. Clark Strategies for the Thermodynamic Characterization of Linked Binding/Local Folding Reactions Within the Native State: Application to the LID Domain of Adenylate Kinase from Escherichia coli Travis P. Schrank, W. Austin Elam, Jing Li and Vincent J. Hilser


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Product Details
  • ISBN-13: 9780123812681
  • Publisher: Elsevier Science Publishing Co Inc
  • Publisher Imprint: Academic Press Inc
  • Depth: 25
  • Height: 229 mm
  • No of Pages: 401
  • Series Title: Volume 488 Methods in Enzymology
  • Sub Title: Volume 488
  • Weight: 790 gr
  • ISBN-10: 0123812682
  • Publisher Date: 22 Feb 2011
  • Binding: Hardback
  • Edition: 1
  • Language: English
  • Returnable: Y
  • Spine Width: 0 mm
  • Volume: 488
  • Width: 152 mm


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Biothermodynamics, Part C: Volume 488(Volume 488 Methods in Enzymology)
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