General Description of the Series:The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents:
General Methodologies:A.A. Russo, Purification and Reconstitution of Cyclin-Dependent Kinase 2 in Four States of Activity.H. Wilhelm, S.S.L. Andersen, and E. Karsenti, Purification of Recombinant Cyclin B1/Cdc2 Kinase from Xenopus Egg Extracts.K.A. Resing and N.G. Ahn, Protein Phosphorylation Analysis by Electrospray Ionization Mass Spectrometry.C. Norbury and S. Moreno, Cloning Cell Cycle Regulatory Genes by Transcomplementation in Yeast.P.D. Adams, P. Lopez, W.R. Sellers, and W.G. Kaelin, Jr., Fluorescence-Activated Cell Sorting of Transfected Cells.N.J.C. Lamb and A. Fernandez, Microinjection of Antibodies into Mammalian Cells.K.D. Lustig, P.T. Stukenberg, T.J. McGarry, R.W. King, V.L. Cryns, P.E. Mead, L.I. Zon, J. Yuan, and M.W. Kirschner, Small Pool Expression Screening: Identification of Genes Involved in Cell Cycle Control, Apoptosis, and Early Development.J. Pines, Localization of Cell Cycle Regulators by Immunofluorescence.L. Meijer and S.-H. Kim, Chemical Inhibitors of Cyclin-Dependent Kinases.D.E. Bassett, Jr., M. Boguski, and P. Hieter, Identifying Human Homologs of Cell Cycle Genes using dbEST and XREFdb.C. Bai and S.J. Elledge, Gene Identification Using the Yeast Two-Hybrid System.Cell Cycle Regulators in Mammalian Systems:S. Freundlieb, U. Baron, A.L. Bonin, M. Gossen, and H. Bujard, Use of Tetracycline-Controlled Gene Expression Systems to Study Mammalian Cell Cycle.R.J. Sheaff, Regulation of Mammalian Cyclin-Dependent Kinase 2.D.E. Phelps and Y. Xiong, Assay for Activity of Mammalian Cyclin D-Dependent Kinases CDK4 and CDK6.J.R. Nevins, J. DeGregori, L. Jakoi, and G. Leone, Functional Analysis of E2F Transcription Factor.L. Stepanova, X. Leng, and J.W. Harper, Analysis of Mammalian Cdc37, A Protein Kinase Targeting Subunit of Heat Shock Protein 90.B.D. Dynlacht, C. Ngwu, J. Winston, E.C. Swindell, S.J. Elledge, E. Harlow, and J.W. Harper, Purification and Analysis of CIP/KIP Proteins. L. Jayaraman, E. Freulick, and C. Prives, Functional Dissection of the p53 Tumor Suppressor Protein.R.P. Fisher, Reconstitution of Mammalian CDK-Activating Kinase.A.M. Fry and E.A. Nigg, Characterization of Mammalian NIMA-Related Kinases.R.Y.C. Poon, Generation of Phosphorylated Cyclin-Dependent Kinase 2 and Functional Characterization of Thrionine-160-Specific Phosphatase KAP.T. Nishimoto, Isolation and Characterization of Temperature-Sensitive Mammalian Cell Cycle Mutants.Cell Cycle Control in Yeast and Fungal Systems:M. Funk, Use of Conditional Promoters in Budding Yeast.S.B. Haase and D.J. Lew, Flow Cytometric Analysis of DNA Content in Budding Yeast.L.L. Breeden, Factor Synchronization of Budding Yeast.L.H. Johnston and A.L. Johnson, Elutriation of Budding Yeast.N. Valtz and M. Peter, Functional Analysis of FAR1 in Yeast.R. Verma, Y. Chi, and R.J. Deshaies, Cell-Free Ubiquitination of Cell Cycle Regulators in Budding Yeast Extracts.C. Santocanale and J.F.X. Diffley, Genomic Footprinting of Budding Yeast Replication Origins during Cell Cycle.J. Campbell, Characterization of Budding Yeast Cdc7 Protein Kinase.Y. Sanchez, Z. Zhou, M. Huang, B.E. Kemp, and S.J. Elledge, Analysis of Budding Yeast Protein Kinases Controlled by DNA Damage.D. Lydall and T. Weinert, Use of cdc-13-1-induced DNA Damage to Study Effects of Checkpoint Genes on DNA Damage Processing.A.F. Straight and A.W. Murray, Spindle Assembly Checkpoint in Budding Yeast.P. Fantes, Genetic Analysis of DNA Replication in Fission Yeast.K. Nabeshima, S. Saitoh, and M. Yanagida, Use of Green Fluorescent Protein for Intracellular Protein Localization in Living Fission Yeast Cells.R.J. Edwards and A.M. Carr, Analysis of Radiation-Sensitive Mutants of Fission Yeast.M.K. Balasubramanian, D. McCollum, and K.L. Gould, Cytokinesis in Fission Yeast Schizosaccharomyces pombe.K. Shiozaki and P. Russell, Stress-Activated Protein Kinase Pathway in Cell Cycle Control of Fission Yeast.X.S. Ye, G. Xu, R.R. Fincher, and S.A. Osmani, Characterization of NIMA Protein Kinase in Aspergillus nidulans.Analysis of Cell Cycle Regulators in Oocyte, Egg, and Embryonic Systems:M.A. Madine and D. Coverley, Xenopus Replication Assays.J.P.J. Chong, P. Thömmes, A. Rowles, H.M.H. Mahbubani, and J.J. Blow, Characterization of Xenopus Replication Licensing System.A. Kumagai and W.G. Dunphy, Regulation of Xenopus Cdc25 Protein.R.-H. Chen and A. Murray, Characterization of Spindle Assembly Checkpoint in Xenopus Egg Extracts.M.S. Murakami and G.F. Vande Woude, Mechanisms of Xenopus Oocyte Maturation.S. Kornbluth, Apoptosis in Xenopus Egg Extracts.J.V. Ruderman, V. Sudakin, and A. Hershko, Preparation of Clam Oocyte Extracts for Cell Cycle Studies.á. Tavares and D.M. Glover, Mitotic Protein Kinases in Drosophila Embryos.Author Index.Subject Index.