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Chemical and Genomic Methods in Nucleic Acid Biology: Volume 705(Volume 705 Methods in Enzymology)

Chemical and Genomic Methods in Nucleic Acid Biology: Volume 705(Volume 705 Methods in Enzymology)

          
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About the Book

Chemical and Genomic Methods in Nucleic Acid Biology, Volume 704 highlights new advances in the field of nucleic acids, with this new volume presenting interesting chapters written by an international board of authors. Specific chapters in this new release include A real-time FRET-based biochemical assay for DNA deaminase enzymology and inhibition, DEER spectroscopy to probe DNA wrapping by protein complexes, PAR-dCLIP: Enhancing PhotoActivatable Ribonucleoside analog-enhanced CrossLinking and Immunoprecipitation through capture of bound 5’ terminal RNA fragments, Site-specific targeting of transgene cDNA insertion, Simultaneous Profiling of the RNA Targets of Two RNA-Binding Proteins Using TRIBE-STAMP, and much more. Additional chapters cover Ensemble FRET Approach to Directly and Continuously Monitor Protein? DNA Interactions, Utilizing nuclear extracts to characterize protein-DNA interactions at the single-molecule level, RNA-Guided Protease Activation in CRISPR-Cas, Activity-based profiling of RNA modifying enzymes, Purification of Cas9 and Cas12a for Peptide-Assisted Genome Editing, Studying the intersection of nucleoside modifications and SARS-CoV-2 RNA-dependent RNA transcription using an in vitro reconstituted system, and more.

Table of Contents:
MIE 704: Chemical and genomic methods in nucleic acid biology 1. A real-time FRET-based biochemical assay for DNA deaminase enzymology and inhibition Hideki Aihara 2. DEER spectroscopy to probe DNA wrapping by protein complexes Edwin Antony 3. PAR-dCLIP: Enhancing PhotoActivatable Ribonucleoside analog-enhanced CrossLinking and Immunoprecipitation through capture of bound 5’ terminal RNA fragments Manuel Ascano 4. Site-specific targeting of transgene cDNA insertion Kathleen collins 5. Simultaneous Profiling of the RNA Targets of Two RNA-Binding Proteins Using TRIBE-STAMP Mathieu Flamand 6. Ensemble FRET Approach to Directly and Continuously Monitor Protein?DNA Interactions Mark Hedglin 7. Utilizing nuclear extracts to characterize protein-DNA interactions at the single-molecule level Bennett Van Houten 8. RNA-Guided Protease Activation in CRISPR-Cas Ailong Ke 9. Activity-based profiling of RNA modifying enzymes Ralph Kleiner 10. Purification of Cas9 and Cas12a for Peptide-Assisted Genome Editing Rahul M. Kohli 11. Studying the intersection of nucleoside modifications and SARS-CoV-2 RNA-dependent RNA transcription using an in vitro reconstituted system Kristin Koutmou 12. Measuring protein stoichiometry with single-molecule imaging in cell-free extracts Joseph Loparo 13. Simultaneous probing of transcription, G-quadruplex, and R-loop Sua Myong 14. A user guide to RT-based mapping of RNA modifications Sigrid Nachtergaele 15. AP Endonuclease 1: Biological updates and advances in activity analysis Robert W. Sobol 16. Strand-specific PCR-competitive replication and adduct bypass assay for assessing how DNA adducts perturb DNA replication in mammalian cells Yinsheng Wang 17. Expression, purification, and biochemical analysis of the RNA-DNA Hybrid Helicase Sen1/SETX from Chaetomium thermophilum R. Scott Williams


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Product Details
  • ISBN-13: 9780443297243
  • Publisher: Elsevier Science Publishing Co Inc
  • Publisher Imprint: Academic Press Inc
  • Height: 229 mm
  • No of Pages: 496
  • Series Title: Volume 705 Methods in Enzymology
  • Weight: 920 gr
  • ISBN-10: 044329724X
  • Publisher Date: 24 Oct 2024
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Sub Title: Volume 705
  • Width: 152 mm


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