Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area.

Table of Contents:
Contents of Volume 63. Contributors. Preface to the Third Edition. Preface to the Second Edition. Preface to the First Edition. Cytogenetics and Molecular Genetics: Sorting of Plant Chromosomes, J. Dolezel, M.A. Lysák, M. Kubaláková, H. Simková, J. Macas, and S. Lucretti. Quantitative DNA Fiber Mapping, H.-U.G. Weier. Primed in Situ Labeling, J. Hindkjaer, L. Bolund, and Steen Kolvraa. Measurements of Telomere Length on Individual Chromosomes by Image Cytometry, S.S.S. Poon and P.M. Lansdorp. Detection of Chromosome Translocation Products in Single Interphase Cell Nuclei, J. Fung, S. Munné, and H.-U.G. Weir. Cell Function and Differentiation: Analysis of Mitochondria by Flow Cytometry, M. Poot and R.H. Pierce. Analysis of RNA Synthesis by Cytometry, P.O. Jensen, J. Larsen, and J.K. Larsen. Flow Cytometry of Erythropoiesis in Culture: Bivariate Profiles of Fetal and Adult Hemoglobin, R.M. Böhmer. Flow Cytometric Analysis of Human Hemopoietic Progenitor Differentiation by Assessing Cell Division Rate and Phenotypic Profile, L. Pierelli, G. Scambia, and A. Fattorossi. Experimental Oncology: Cytometry of Antitumor Drug-Intracellular Target Interactions, P.J. Smith and M. Wiltshire. Monitoring of Cellular Resistance to Cancer Chemotherapy: Drug Retention and Efflux, A. Krishan. Resistance of Tumor Cells to Chemo- and Radiotherapy Modulated by the Three-Dimensional Architecture of Solid Tumors and Spheroids, R.E. Durand and P.L. Olive. Analysis of DNA Damage in Individual Cells, P.L. Olive, R.E. Durand, J.P. Banáth, and P.J. Johnston. Cytometric Methods to Analyze Ionizing-Radiation Effects, W.D. Wright, I. Lagroye, P. Zhang, R.S. Malyapa, and J.L. Roti Roti. Clinical Oncology: Multiparameter Data Acquisition and Analysis of Leukocytes by Flow Cytometry, C.C. Stewart and S.J. Stewart. Immunophenotyping of Hemotological Malignancies by Laser Scanning Cytometry, R.J. Clatch. Immunophenotyping of Acute Leukemia: Utility of CD45 for Blast Cell Identification, J.-P. Vial and F. Lacombe. Cell Proliferation Markers in Human Solid Tumors: Assessing Their Impact in Clinical Oncology, M.G. Daidone, A. Costa, and R. Silvestrini. Detection of Minimal Residual Disease, A. Deptala and S.P. Mayer. Analysis of Human Tumors by Laser Scanning Cytometry, W. Corczyca, A. Deptala, E. Bedner, X. Li, M.R. Melamed, and Z. Darzynkiewicz. Laser Cytometry of Human Tissues and Tumors: Proliferation and Therapeutic Applications, D.A. Rew. Prediction and Precise Diagnosis of Diseases by Data Pattern Analysis in Multiparameter Flow Cytometry: Melanoma, Juvenile Asthma, and Human Immunodeficiency Virus Infection, G. Valet, H. Kahle, F. Otto, E. Bräutigam, and L. Kestens. Microorganisms and Infectious Diseases: Flow Cytometric Analysis of Microorganisms, S.A. Sincock and J.P. Robinson. Staining and Measurement of DNA in Bacteria, H.B. Steen. Flow Cytometric Monitoring of Bacterial Susceptibility to Antibiotics, M. Walberg and H.B. Steen. Flow Cytometry for Evaluation and Investigation of Human Immunodeficiency Virus Infection, T.W. McCloskey. Index. Volumes in Series.