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Developmental Biology Protocols: Volume II(136 Methods in Molecular Biology)

Developmental Biology Protocols: Volume II(136 Methods in Molecular Biology)

          
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About the Book

Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.

Table of Contents:
Developmental Biology Protocols.- Organogenesis.- Drosophila as a Genetic Tool to Define Vertebrate Pathway Players.- Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis.- Analysis of Mammary Gland Morphogenesis.- Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro.- Craniofacial Development and Patterning.- Craniofacial Skeletel Morphogenesis In Vitro.- Skeletal Morphogenesis.- Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration.- Retroviral Infection of T-Cell Precursors in Thymic Organ Culture.- Assay for the Isolation of Hepatogenic Factors.- Skin Morphogenesis.- Apoptosis in Development.- Methods to Detect Patterns of Cell Death in Drosophila.- Abnormal Development and Teratology.- Mechanisms of Neurulation.- Neurulation and Neural Tube Closure Defects.- Neural Tube Defects.- Experimental Manipulation and Morphometric Analysis of Neural Tube Development.- Isolation of Neuroepithelium and Formation of Minispheres.- Examination of Normal and Abnormal Placentation in the Mouse.- Palatal Dysmorphogenesis.- Palatal Dysmorphogenesis.- Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities.- Assessment of Laterality Defects in Rodent Embryos.- Cardiac Morphogenesis and Dysmorphogenesis I. Normal Development.- Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart.- Biologically Based Risk Assessment Models for Developmental Toxicity.- Screening and Mapping of Novel Genes and Mutations.- Positional Cloning.- Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse.- PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes.- Analysis of mRNA ExpressionDuring Preimplantation Development.- Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos.- HPLC-Based mRNA Differential Display.- Production of Transgenic Drosophila.- Transgenic Manipulation of the Sea Urchin Embryo.- Transgenic Zebrafish.- Production of Avian Chimeras and Germline Transmission.- Incorporation of Genetically Modified Cells in Chicken Chimeras.- Long-Term Culture of Chicken Blastodermal Cells (CBCs) and Selection of Transfected CBCs Using Antibiotic Resistance.- Nuclear Transplantation and Cloning in Mammals.- Production of Transgenic Mice.- Production of Transgenic Mice with Yeast Artificial Chromosomes.- Gene-Targeting Strategies.- Chimeric Animals and GermlineTransmission.- Conditional Gene Knockout Using Cre Recombinase.- Application of Cre/loxP in Drosophila.


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Product Details
  • ISBN-13: 9780896035751
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: Annotated edition
  • Language: English
  • Returnable: N
  • Spine Width: 32 mm
  • Weight: 892 gr
  • ISBN-10: 0896035751
  • Publisher Date: 17 Jan 2000
  • Binding: Paperback
  • Height: 244 mm
  • No of Pages: 505
  • Series Title: 136 Methods in Molecular Biology
  • Sub Title: Volume II
  • Width: 170 mm


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