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Electron Crystallography of Soluble and Membrane Proteins

Electron Crystallography of Soluble and Membrane Proteins

          
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About the Book

1. Introduction to Electron Crystallography

Werner Kühlbrandt

2. Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis

Kutti R. Vinothkumar, Patricia C. Edwards, and Joerg Standfuss

3. Two-Dimensional Crystallization of Membrane Proteins by Reconstitution through Dialysis

Matthew C. Johnson, Tina M. Dreaden, Laura Y. Kim, Frederik Rudolph, Bridgette Barry, and Ingeborg Schmidt-Krey

4. Monolayer Two-Dimensional Crystallization

Luc Lebeau and Catherine Vénien-Bryan

5. Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples

Tina M. Dreaden, Maureen Metcalfe, Laura Y. Kim, Matthew C. Johnson, Bridgette A. Barry, and Ingeborg Schmidt-Krey

6. Low Dose Techniques and Cryo-Electron Microscopy

Yoshinori Fujiyoshi

7. Grid Preparation for Cryo-Electron Microscopy

Nobuhiko Gyobu

8. Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins

Agustin Avila-Sakar, Xueming Li, Shawn Q. Zheng, and Yifan Cheng

9. The Collection of High-Resolution Electron Diffraction Data

Tamir Gonen

10. Image Processing of 2D Crystal Images

Marcel Arheit, Daniel Castaño-Diéz, Raphael Thierry, Bryant R. Gipson, Xiangyan Zeng, and Henning Stahlberg

11. Merging of Image Data in Electron Crystallography

Marcel Arheit, Daniel Castaño-Diéz, Raphael Thierry, Priyanka Abeyrathne, Bryant R. Gipson, and Henning Stahlberg

12. Evaluation of Electron Crystallographic Data From Images of Two-Dimensional Crystals

Vinzenz M. Unger

13. Modeling, Docking, and Fitting of Atomic Structures to 3D Maps from Cryo-Electron Microscopy

Gregory S. Allen and David L. Stokes

14. Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement

Goragot Wisedchaisri and Tamir Gonen

15. High-Throughput Methods for Electron Crystallography

David L. Stokes, Iban Ubarretxena-Belandia, Tamir Gonen, and Andreas Engel

16. Automated Grid Handling and Image Acquisition for Two-Dimensional Crystal Screening

Anchi Cheng

17. Automation of Data Acquisition in Electron Crystallography

Anchi Cheng

18. Automation of Image Processing in Electron Crystallography

Marcel Arheit, Daniel Castaño-Diéz, Raphael Thierry, Bryant R. Gipson, Xiangyan Zeng, and Henning Stahlberg

19. Choice and Maintenance of Equipment for Electron Crystallography

Deryck J. Mills and Janet Vonck

20. Future Developments in Instrumentation for Electron Crystallography

Kenneth H. Downing

21. Tubular Crystals and Helical Arrays: Structural Determination of HIV-1 Capsid Assemblies Using Iterative Helical Real-Space Reconstruction

Peijun Zhang, Xin Meng, and Gongpu Zhao

22. Single Particle Electron Microscopy

Wilson C.Y. Lau and John L. Rubinstein

23. Electron Tomography of Paracrystalline 2D Arrays

Hanspeter Winkler, Shenping Wu, and Kenneth A. Taylor

24. High-Resolution Imaging of 2D Outer Membrane Protein F (OmpF) Crystals by Atomic Fo


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Product Details
  • ISBN-13: 9781493958764
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: Softcover reprint of the original 1st ed. 2013
  • Language: English
  • Returnable: Y
  • Spine Width: 31 mm
  • Weight: 1028 gr
  • ISBN-10: 1493958763
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 586
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm


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