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Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice

Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice

          
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About the Book

Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice is an indispensable companion to the biomedical literature. This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature.  Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. This unique book will be an invaluable resource for students, technicians and researchers in all areas of biomedicine.   Allows the reader to develop the necessary skills to properly evaluate research articles Coverage of over 30 commonly-used techniques in the biomedical sciences Global approach and application, with contributions from leading experts in diverse fields

Table of Contents:
List of contributors ix Foreword xi Preface xiii Acknowledgements xvii Introduction xix Section A - Basic principles 1 1 Philosophy of science 3 James Ladyman 2 Ingredients of experimental design 9 Nick Colegrave 3 Statistics: a journey that needs a guide 17 Gordon Drummond Section B - Cell and molecular 27 4 Organ bath pharmacology 29 Emma Robinson 5 Small vessel myography 39 Tomoko Kamishima and John M Quayle 6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49 Scott H Randell 7 Electron microscopy (TEM and SEM) 59 Paul Verkade 8 Fluorescence microscopy 67 Mark Jepson 9 Intracellular ‘sharp’ microelectrode recording 77 Helena C Parkington and Harold A Coleman 10 Single electrode voltage-clamp (SEVC) 85 Harold A Coleman and Helena C Parkington 11 Patch clamp recording 95 Neil Bannister and Phil Langton 12 Production of antibodies 105 Elek Molnár 13 Immunocytochemistry and immunohistochemistry 117 Elek Molnár 14 Immunoprecipitation (IP) 129 David Bates 15 Immunoblotting (western) 137 Samantha F. Moore, Joshua S. Savage and Ingeborg Hers 16 Applications of green fluorescent protein (GFP) 147 Mark Jepson 17 Fluorescent measurement of ion activity in cells 153 Helen Kennedy 18 Detection of exocytosis – real time 163 Anja Teschemacher 19 Viral vector transgenesis 173 Anja Teschemacher 20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179 Lucy F. Donaldson 21 In situ hybridisation (ISH) 187 Lucy F. Donaldson 22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193 Allison Fulford 23 Transcriptome analysis: microarrays 203 Charles Hindmarch 24 Experimental proteomics 215 Thierry Le Bihan Section C - In vivo / integrative 229 25 Behavioural methodology 231 Emma Robinson 26 Genetically modified mouse models 241 Nina Balthasar 27 Wireless recording of cardiovascular signals 247 Julian FR Paton and Fiona D McBryde 28 Electrical stimulation methods 253 Jon Wakerley 29 Extracellular recording 261 Jon Wakerley 30 Antidromic identification 271 Jon Wakerley 31 Event-triggered averaging, including spike-triggered averaging 279 Richard Apps 32 Axonal transport tracing of CNS pathways 285 John Crabtree 33 Cardiovascular methods: general considerations for human studies 291 Erica A Wehrwein and Michael J Joyner 34 Measuring cardiac output in humans 299 Erica A Wehrwein and Michael J Joyner 35 Measuring peripheral blood flow in humans 311 Erica A Wehrwein and Michael J Joyner Index 319


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Product Details
  • ISBN-13: 9781119959960
  • Publisher: John Wiley and Sons Ltd
  • Publisher Imprint: Wiley-Blackwell
  • Depth: 19
  • Height: 254 mm
  • No of Pages: 352
  • Series Title: English
  • Sub Title: Recognising and Interpreting Best Practice
  • Width: 178 mm
  • ISBN-10: 1119959969
  • Publisher Date: 23 Nov 2012
  • Binding: Hardback
  • Edition: HAR/PSC
  • Language: English
  • Returnable: N
  • Spine Width: 25 mm
  • Weight: 717 gr


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