Part I: Steady-State Fluorescence Spectroscopy (SSFS)
1. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra
Adam Matthew Gilmore
2. Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions
Nicholas G. James and David M. Jameson
3. Quantitative Fluorescence Spectral Analysis of Protein Denaturation
Ivo H.M. van Stokkum and Sergey P. Laptenok
4. High-Pressure Fluorescence Applications
Mariano Dellarole and Catherine A. Royer
Part II: Time-Resolved Fluorescence Spectroscopy (TRFS)
5. Frequency Domain Fluorometry: Theory and Application
Carissa M. Vetromile and David M. Jameson
6. Polar Plot Representation of Time-Resolved Fluorescence
John Paul Eichorts, Kai wen Teng, and Robert M. Clegg
7. Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics
Tomer Orevi, Eitan Lerner, Gil Rahamim, Dan Amir, and Elisha Haas
8. MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes
Patrik R. Callis and Jose R. Tusell
9. Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins
Dmitri Toptygin
10. Global Analysis of Time-Resolved Fluorescence Data
Anatoli V. Digris, Eugene G. Novikov, Victor V. Skakun, and Vladimir V. Apanasovich
11. Nanometrology
David J.S. Birch and Philip Yip
12. Upconversion Spectrophotofluorometry
Arianna Biesso, Jianhua Xu, and Jay R. Knutson
13. Subpicosecond Kerr-Gate Spectrofluorometry
Sergey P. Laptenok, Patrick Nürnberger, Andras Lukacs, and Marten H. Vos
14. Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay
Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, and Fumio Tanaka
Part III: Fluorescent Probe Development (FPD)
15. Biosynthetic Incorporation of Trp Analogs in Proteins
Jaap Broos
16. Optimization of Fluorescent Proteins
Daphne S. Bindels, Joachim Goedhart, Mark A. Hink, Laura van Weeren, Linda Joosen, and Theodorus W.J. Gadella, Jr.
17. Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family
Zeinab Darwich, Andrey S. Klymchenko, and Yves Mély
Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP)
18. Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope
Ranhua Xiong, Hendrik Deschout, Jo Demeester, Stefaan C. De Smedt, and Kevin Braeckmans
Part V: Fluorescence Microscopy: Förster Resonance Energy Transfer Imaging (FRETim)
19. A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging
Clemens F. Kaminski, Eric. J. Rees, and Gabriele S. Kaminski Schierle
Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM)
20. Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors
Roland Hartig, Yury Prokazov, Evgeny Turbin, and Werner Zuschratter
21. Global Analysis of FRET- FLIM Data in Live Plant Cells
Sergey P. Laptenok, Joris J. Snellenburg, Christoph A. Bücherl, Kai R. Konrad, and Jan Willem Borst
22. Time-Resolved Fluorescence Anisotropy Imaging
Klaus Suhling, James Levitt, and Pei-Hua Chung
23. Multi-Modal Fluo
