Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications of High Performance Liquid Chromatography uses real-life examples likely to be found within a forensic science laboratory to explain HPLC from a forensic perspective.
Focusing chiefly on the reverse phase HPLC mode of separation, this volume examines:
- The history of HPLC and the theory behind the separation process
- The requirements for successful analysis and best practice tips
- The modes of separation and detection most appropriate for forensic science applications
- HPLC method development and evaluation
- The quality aspects of laboratory operation
- Troubleshooting HPLC systems and analyses
- Applications of HPLC within the field of forensic science
Designed as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is also an invaluable guide for those in the early stages of their forensic analysis careers.
An instructor’s manual with lecture slides, test bank, objectives, and exercises is available with qualifying course adoption
Table of Contents:
Introduction to HPLC
Introduction
The History of Chromatography
Basic Instrumentation
Further Reading
Basic Principles of HPLC
Theory of Chromatography
Chemical Bonding and Polarity
Mobile Phase Preparation, the Use of Buffers, and Sample Preparation
Mobile Phase Preparation
Buffers
Sample and Standard Preparation
Modes of Separation
Introduction
Reverse Phase HPLC
Ion Exchange
Normal Phase HPLC
Detection Systems
Introduction
Theory
UV/Vis Detectors versus Diode Array Detectors
Electrochemical Detectors
Mass Spectrometry
Method Development in Reversed Phase HPLC
Introduction
Sample Composition
Which Column Will Affect the Separation?
Choosing a Mobile Phase
Automated Method Development
Method Development Strategy
Method Development Worked Example
System Suitability
Introduction
System Suitability
System Performance
Chromatographic Suitability
Qualification, Validation, and Verification
Introduction
Valid Analytical Measurement
Estimation of Uncertainty of Measurement, Sources of Error, and Statistical Evaluation
Instrument Qualification
Method Validation
Method Verification
Quality Systems
Introduction
What Is Quality?
Introduction to Quality Assurance
How Do We Ensure Quality in Our Laboratories?
A Quality Management System
Accreditation
Auditing
Troubleshooting HPLC Systems
Introduction
Troubleshooting
Tool Kit and Spare Parts
Preventative Maintenance
Column Care
Forensic Applications of HPLC
Introduction
Drug Analysis
Toxicological Analysis
Colour Analysis
Analysis of Explosives
Food and the Environment
Glossary
Index
