Voet, Voet, and Pratt’s Fundamentals of Biochemistry, challenges students to better understand the chemistry behind the biological structure and reactions occurring in living systems. The Third Edition continues this tradition, and additionally incorporates coverage of recent research and an expanded focus on preparing and supporting students throughout the course. With the addition of new conceptual assessment content to WileyPLUS, students have the opportunity to assess their conceptual understanding of key introductory biochemistry concepts and retrain themselves on their misconceptions.

Table of Contents:
Preface xviii Acknowledgments xxi Instructor and Student Resources xxiii Guide to Media Resources xxv PART I INTRODUCTION 1 Introduction to the Chemistry of Life 1 1 The Origin of Life 2 2 Cellular Architecture 5 3 Thermodynamics 11 E. Life Obeys the Laws of Thermodynamics 17 BOX 1-1 PATHWAYS OF DISCOVERY BOX 1-2 PERSPECTIVES IN BIOCHEMISTRY 2 Water 22 1 Physical Properties of Water 23 2 Chemical Properties of Water 30 BOX 2-1 BIOCHEMISTRY IN HEALTH AND DISEASE PART II BIOMOLECULES 3 Nucleotides, Nucleic Acids, and Genetic Information 39 1 Nucleotides 40 2 Introduction to Nucleic Acid Structure 43 3 Overview of Nucleic Acid Function 47 4 Nucleic Acid Sequencing 50 5 Manipulating DNA 59 BOX 3-1 PATHWAYS OF DISCOVERY BOX 3-2 PERSPECTIVES IN BIOCHEMISTRY BOX 3-3 PERSPECTIVES IN BIOCHEMISTRY 4 Amino Acids 74 1 Amino Acid Structure 74 2 Stereochemistry 82 3 Amino Acid Derivatives 86 BOX 4-1 PATHWAYS OF DISCOVERY BOX 4-2 PERSPECTIVES IN BIOCHEMISTRY BOX 4-3 PERSPECTIVES IN BIOCHEMISTRY 5 Proteins: Primary Structure 91 1 Polypeptide Diversity 91 2 Protein Purification and Analysis 94 3 Protein Sequencing 104 4 Protein Evolution 114 BOX 5-1 PATHWAYS OF DISCOVERY 6 Proteins:Three-Dimensional Structure 125 1 Secondary Structure 127 2 Tertiary Structure 140 3 Quaternary Structure and Symmetry 154 4 Protein Stability 156 5 Protein Folding 161 BOX 6-1 PATHWAYS OF DISCOVERY BOX 6-2 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 6-3 PERSPECTIVES IN BIOCHEMISTRY BOX 6-4 PERSPECTIVES IN BIOCHEMISTRY 7 Protein Function: Myoglobin and Hemoglobin, Muscle Contraction, and Antibodies 176 1 Oxygen Binding to Myoglobin and Hemoglobin 177 2 Muscle Contraction 197 3 Antibodies 209 BOX 7-1 PERSPECTIVES IN BIOCHEMISTRY BOX 7-2 PATHWAYS OF DISCOVERY BOX 7-3 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 7-4 PATHWAYS OF DISCOVERY BOX 7-5 PERSPECTIVES IN BIOCHEMISTRY 8 Carbohydrates 219 1 Monosaccharides 220 2 Polysaccharides 226 3 Glycoproteins 234 BOX 8-1 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 8-2 PERSPECTIVES IN BIOCHEMISTRY BOX 8-3 BIOCHEMISTRY IN HEALTH AND DISEASE 9 Lipids and Biological Membranes 245 1 Lipid Classification 246 2 Lipid Bilayers 260 3 Membrane Proteins 263 4 Membrane Structure and Assembly 269 BOX 9-1 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 9-2 PATHWAYS OF DISCOVERY BOX 9-3 BIOCHEMISTRY IN HEALTH AND DISEASE 10 Membrane Transport 295 1 Thermodynamics of Transport 296 2 Passive-Mediated Transport 297 3 Active Transport 311 BOX 10-1 PERSPECTIVES IN BIOCHEMISTRY BOX 10-2 PERSPECTIVES IN BIOCHEMISTRY BOX 10-3 BIOCHEMISTRY IN HEALTH AND DISEASE PART III ENZYMES 11 Enzymatic Catalysis 322 1 General Properties of Enzymes 323 2 Activation Energy and the Reaction Coordinate 328 3 Catalytic Mechanisms 330 4 Lysozyme 339 5 Serine Proteases 347 BOX 11-1 PERSPECTIVES IN BIOCHEMISTRY BOX 11-2 PERSPECTIVES IN BIOCHEMISTRY BOX 11-3 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 11-4 BIOCHEMISTRY IN HEALTH AND DISEASE 12 Enzyme Kinetics, Inhibition, and Control 363 1 Reaction Kinetics 364 2 Enzyme Inhibition 377 3 Control of Enzyme Activity 386 4 Drug Design 394 BOX 12-1 PERSPECTIVES IN BIOCHEMISTRY BOX 12-2 PATHWAYS OF DISCOVERY BOX 12-3 PERSPECTIVES IN BIOCHEMISTRY BOX 12-4 BIOCHEMISTRY IN HEALTH AND DISEASE 13 Biochemical Signaling 405 1 Hormones 406 2 Receptor Tyrosine Kinases 412 3 Heterotrimeric G Proteins 428 4 The Phosphoinositide Pathway 436 BOX 13-1 PATHWAYS OF DISCOVERY BOX 13-2 PERSPECTIVES IN BIOCHEMISTRY BOX 13-3 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 13-4 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 13-5 BIOCHEMISTRY IN HEALTH AND DISEASE PART IV METABOLISM 14 Introduction to Metabolism 448 1 Overview of Metabolism 449 2 “High-Energy” Compounds 459 3 Oxidation–Reduction Reactions 469 4 Experimental Approaches to the Study of Metabolism 475 BOX 14-1 PERSPECTIVES IN BIOCHEMISTRY BOX 14-2 PERSPECTIVES IN BIOCHEMISTRY BOX 14-3 PATHWAYS OF DISCOVERY BOX 14-4 PERSPECTIVES IN BIOCHEMISTRY 15 Glucose Catabolism 485 1 Overview of Glycolysis 486 2 The Reactions of Glycolysis 489 3 Fermentation:The Anaerobic Fate of Pyruvate 504 4 Regulation of Glycolysis 510 5 Metabolism of Hexoses Other than Glucose 516 6 The Pentose Phosphate Pathway 520 BOX 15-1 PATHWAYS OF DISCOVERY BOX 15-2 PERSPECTIVES IN BIOCHEMISTRY BOX 15-3 PERSPECTIVES IN BIOCHEMISTRY BOX 15-4 BIOCHEMISTRY IN HEALTH AND DISEASE 16 Glycogen Metabolism and Gluconeogenesis 530 1 Glycogen Breakdown 532 2 Glycogen Synthesis 540 3 Control of Glycogen Metabolism 545 4 Gluconeogenesis 552 5 Other Carbohydrate Biosynthetic Pathways 560 BOX 16-1 PATHWAYS OF DISCOVERY BOX 16-2 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 16-3 PERSPECTIVES IN BIOCHEMISTRY BOX 16-4 PERSPECTIVES IN BIOCHEMISTRY 17 Citric Acid Cycle 566 1 Overview of the Citric Acid Cycle 567 2 Synthesis of Acetyl-Coenzyme A 570 3 Enzymes of the Citric Acid Cycle 576 4 Regulation of the Citric Acid Cycle 583 5 Reactions Related to the Citric Acid Cycle 588 BOX 17-1 PATHWAYS OF DISCOVERY BOX 17-2 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 17-3 PERSPECTIVES IN BIOCHEMISTRY 18 Electron Transport and Oxidative Phosphorylation 596 1 The Mitochondrion 597 2 Electron Transport 600 3 Oxidative Phosphorylation 618 4 Control of Oxidative Metabolism 631 BOX 18-1 PERSPECTIVES IN BIOCHEMISTRY BOX 18-2 PATHWAYS OF DISCOVERY BOX 18-3 PERSPECTIVES IN BIOCHEMISTRY  BOX 18-4 PERSPECTIVES IN BIOCHEMISTRY  BOX 18-5 BIOCHEMISTRY IN HEALTH AND DISEASE 19 Photosynthesis 640 1 Chloroplasts 641 2 The Light Reactions 645 3 The Dark Reactions 663 BOX 19-1 PERSPECTIVES IN BIOCHEMISTRY 20 Lipid Metabolism 677 1 Lipid Digestion, Absorption, and Transport 678 2 Fatty Acid Oxidation 685 3 Ketone Bodies 698 4 Fatty Acid Biosynthesis 701 5 Regulation of Fatty Acid Metabolism 711 6 Synthesis of Other Lipids 714 7 Cholesterol Metabolism 721 BOX 20-1 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 20-2 PATHWAYS OF DISCOVERY  BOX 20-3 PERSPECTIVES IN BIOCHEMISTRY BOX 20-4 BIOCHEMISTRY IN HEALTH AND DISEASE 21 Amino Acid Metabolism 732 1 Protein Degradation 732 2 Amino Acid Deamination 738 3 The Urea Cycle 743 4 Breakdown of Amino Acids 747 5 Amino Acid Biosynthesis 763 6 Other Products of Amino Acid Metabolism 774 7 Nitrogen Fixation 782 BOX 21-1 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 21-2 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 21-3 BIOCHEMISTRY IN HEALTH AND DISEASE 22 Mammalian Fuel Metabolism: Integration and Regulation 791 1 Organ Specialization 792 2 Hormonal Control of Fuel Metabolism 799 3 Metabolic Homeostasis:The Regulation of Energy Metabolism, Appetite, and Body Weight 804 4 Disturbances in Fuel Metabolism 809 BOX 22-1 PATHWAYS OF DISCOVERY  PART V GENE EXPRESSION AND REPLICATION 23 Nucleotide Metabolism 817 1 Synthesis of Purine Ribonucleotides 818 2 Synthesis of Pyrimidine Ribonucleotides 824 3 Formation of Deoxyribonucleotides 828 4 Nucleotide Degradation 839 BOX 23-1 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 23-2 PATHWAYS OF DISCOVERY 24 Nucleic Acid Structure 848 1 The DNA Helix 849 2 Forces Stabilizing Nucleic Acid Structures 864 3 Fractionation of Nucleic Acids 872 4 DNA–Protein Interactions 874 5 Eukaryotic Chromosome Structure 883 BOX 24-1 PATHWAYS OF DISCOVERY BOX 24-2 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 24-3 PERSPECTIVES IN BIOCHEMISTRY 25 DNA Replication, Repair, and Recombination 893 1 Overview of DNA Replication 894 2 Prokaryotic DNA Replication 896 3 Eukaryotic DNA Replication 910 4 DNA Damage 916 5 DNA Repair 920 6 Recombination 926 BOX 25-1 PATHWAYS OF DISCOVERY BOX 25-2 PERSPECTIVES IN BIOCHEMISTRY BOX 25-3 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 25-4 PERSPECTIVES IN BIOCHEMISTRY BOX 25-5 PERSPECTIVES IN BIOCHEMISTRY 26 Transcription and RNA Processing 942 1 Prokaryotic RNA Transcription 943 2 Transcription in Eukaryotes 952 3 Posttranscriptional Processing 965 BOX 26-1 PERSPECTIVES IN BIOCHEMISTRY BOX 26-2 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 26-3 PATHWAYS OF DISCOVERY 27 Protein Synthesis 985 1 The Genetic Code 986 2 Transfer RNA and Its Aminoacylation 991 3 Ribosomes 1000 4 Translation 1008 5 Posttranslational Processing 1028 BOX 27-1 PERSPECTIVES IN BIOCHEMISTRY BOX 27-2 PERSPECTIVES IN BIOCHEMISTRY BOX 27-3 BIOCHEMISTRY IN HEALTH AND DISEASE 28 Regulation of Gene Expression 1037 1 Genome Organization 1038 2 Regulation of Prokaryotic Gene Expression 1046 3 Regulation of Eukaryotic Gene Expression 1055 4 The Cell Cycle, Cancer, and Apoptosis 1081 BOX 28-1 BIOCHEMISTRY IN HEALTH AND DISEASE BOX 28-2 PERSPECTIVES IN BIOCHEMISTRY BOX 28-3 PERSPECTIVES IN BIOCHEMISTRY APPENDICES Solutions to Problems SP-1 Glossary G-1 Index I-1