Gap Junction Protocols (Hardback) | Released: 21 May 2016

1. Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

Michaël Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken

2. DNA Methylation Analysis of Human Tissue-Specific Connexin Genes

Xiaoming Liu and Canxia Xu

3. Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis

Joost Willebrords, Michaël Maes, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken

4. Immunohisto- and Cytochemistry Analysis of Connexins

Bruno Cogliati, Michaël Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara Crespo Yanguas, and Mathieu Vinken

5. Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells

Miranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, and Brant E. Isakson

6. Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor

Mark Kibschull, Stephen J. Lye, and Oksana Shynlova

7. Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells

Alexander W. Lohman, Adam C. Straub, and Scott R. Johnstone

8. Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis

Stephanie Fowler, Mark Akins, and Steffany A.L. Bennett

9. Scrape Loading/Dye Transfer Assay

Pavel Babica, Iva Sovadinová, and Brad L. Upham

10. Microinjection Technique for Assessment of Gap Junction Function

Michael D. Fridman, Jun Liu, Yu Sun, and Robert M. Hamilton

11. Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling

Elke Decrock, Marijke De Bock, Diego De Baere, Delphine Hoorelbeke, Nan Wang, and Luc Leybaert

12. Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro

Maria Kuzma-Kuzniarska, Clarence Yapp, and Philippa A. Hulley

13. Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging

14. A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells

Joseph P. Stains and Roberto Civitelli

15. Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels