Part I: Gene Disruption
1. Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy
Christoph Sasse and Joachim Morschhäuser
2. Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans
Shantanu Ganguly and Aaron P. Mitchell
3. Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans
Megan D. Lenardon and André Nantel
4. Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi
Olga Zemska and Chad A. Rappleye
5. Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers
Min Su Kim, Seo-Young Kim, Kwang-Woo Jung, and Yong-Sun Bahn
6. Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System
Lorina G. Bakerand and Jennifer K. Lodge
7. Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast
Iran Malavazi and Gustavo Henrique Goldman
8. Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach
Fabrice N. Gravelat, David S. Askew, and Donald C. Sheppard
9. Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers
Chiung-Yu Hung, Hua Zhang Wise, and Garry T. Cole
Part II: Modulation of Gene Expression: RNAi Gene Knockdown
10. RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum
Brian H. Youseff and Chad A. Rappleye
11. RNA Interference in Cryptococcus neoformans
Michael L. Skowyra and Tamara L. Doering
12. Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA
João F. Menino, Agostinho J. Almeida, and Fernando Rodrigues
Part III: Modulation of Gene Expression: Regulatable Promoters
13. Tetracycline-Inducible Gene Expression in Candida albicans
Michael Weyler and Joachim Morschhäuser
14. Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii
Lorina G. Baker and Jennifer K. Lodge
15. Modular Gene Over-Expression Strategies for Candida albicans
Vitor Cabral, Murielle Chauvel, Arnaud Firon, Mélanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Munro, and Christophe d'Enfert
Part IV: Responses to Infection In Vivo
16. Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans
Héctor M. Mora-Montes, Christopher McKenzie, Judith M. Bain, Leanne E. Lewis, Lars P. Erwig, and Neil A.R. Gow
17. Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans
Joanne Gibson, Neil A.R. Gow, and Simon Y.C. Wong
18. Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils
Alieke G. Vonk, Mihai G. Netea, and Bart Jan Kullberg
19. Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions
Torsten Wöllert, Christiane Rollenhagen, George M. Langford, and Paula Sundstrom
