Lorette Javois' timely new 2nd edition of Immunocytochemistry Methods revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization. From Reviews of the First Edition: " a useful, sometimes beautiful compilation of protocols and methods " - FEBS Letters " an extremely useful vade mecum' of comprehensive, detailed recipes, good advice and helpful information, spiral bound in a handy size for laboratory use." - Society for General Microbiology Quarterly

Table of Contents:
Part I. Antibody Preparation: Overview of Antibody Use in Immunocytochemistry, Su-Yau Mao, Lorette C. Javois, and Ute M. Kent. Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration, Ute M. Kent. Purification of Antibodies Using Ion-Exchange Chromatography, Ute M. Kent. Purification of Antibodies Using Affinity Chromatography, Ute M. Kent. Purification of Antibodies Using Protein A-Sepharose and FPLC, Ute M. Kent. Conjugation of Fluorochromes to Antibodies, Su-Yau Mao. Biotinylation of Antibodies, Su-Yau Mao. Part II. Tissue Preparation for Light Microscopic Analyses. Overview of Cell Fixatives and Cell Membrane Permeants, Melissa A. Melan. Preparation of Frozen Sections for Analysis, Gary L. Bratthauer. Processing of Cytological Specimens, Gary L. Bratthauer. Processing of Tissue-Culture Cells, Gary L. Bratthauer. Processing of Tissue Specimens, Gary L. Bratthauer. Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections, Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld. Part III. Light Microscopic Detection Systems. Overview of Fluorochromes, J. Michael Mullins. Direct Immunofluorescent Labeling of Cells, Lorette C. Javois. Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue Culture Cells, Mark C. Willingham. Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells, Mark C. Willingham. Fluorescent Labeling of Surface or Intracellular Antigens in Whole Mounts, Lorette C. Javois. TUNEL Assay for Apoptic Cells, Virginia M. Heatwole. Overview of Fluorescence Analysis with the Confocal Microscope, Liana Harvath. Overview of Laser Microbeam Applications as Related to Antibody Targeting, P. Scott Pine. Overview of Fluorescence Photomicography, Lorette C. Javois and J. Michael Mullins. Overview of Antigen Detection Through Enzymatic Activity, Gary L. Bratthauer. The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods, Gary L. Bratthauer. The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods, Gary L. Bratthauer. Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue, Mark Raffeld and Elaine S. Jaffe. Multiple Antigen Immunostaining Procedures, Tibor Krenacs, Laszlo Krenacs, and Mark Raffeld. ELISA on Attachment-Dependent or Suspension Grown Cells, Julie Ann Brent. Use of Immunogold with Silver Enhancement, Constance Oliver. Part IV. Fluorescence Activated Cell Sorter (FACS) Analyses. Overview of Flow Cytometry and Fluorescent Probes for Cytometry, Robert E. Cunningham. Tissue Disaggregation, Robert E. Cunningham. Indirect Immunofluorescent Labeling of Viable Cells, Robert E. Cunningham. Indirect Immunofluorescent Labeling of Fixed Cells, Robert E. Cunningham. Fluorescent Labeling of DNA, Robert E. Cunningham. Deparaffinization and Processing of Pathological Material, Robert E. Cunningham. Assay for Phagocytosis, Liana Harvath and Douglas A. Terle. Assay for Filamentous Actin, Liana Harvath. Assay for Chemoattractant Binding, Liana Harvath, Robert R. Aksamit, and Robert E. Cunningham. Assay for Oxidative Metabolism, Liana Harvath and Douglas A. Terle. Colloidal Gold Detection Systems for Electron Microscopic Analyses. Fixation and Embedding. Preparation of Colloidal Gold. Conjugation of Colloidal Gold to Proteins. Colloidal Gold/Streptavidin Methods. Pre-Embedding Labeling Methods. Postembedding Labeling Methods. Photography. Overview of Fluorescence Photography. Special Applications. Overview of Fluorescence Analysis with the Confocal Microscope. Overview of Laser Microbeam Applications as Related to Antibody Targeting. Nucleic Acid Immunocytochemistry: In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase- Labeled Centromeric Probe. Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes. Overview of Automated Immunostainers. Overview of Immunohistochemical Approaches to the Differential Diagnosis of Tumors. Index.