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In Vitro Neurotoxicology

In Vitro Neurotoxicology

          
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About the Book

1. In Vitro Neurotoxicology: An Introduction

Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti

Part I: Model Systems

2. Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies

Gennaro Giordano and Lucio G. Costa

3. Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures

Evelyn Tiffany-Castiglioni, Yongchang Qian, Rola Barhoumi, and Ying Zheng

4. Microglia Cell Culture: A Primer for the Novice

Anke Witting and Thomas Möller

5. Neural Stem Cells for Developmental Neurotoxicity Studies

Roshan Tofighi, Michaela Moors, Raj Bose, Wan Norhamidah Wan Ibrahim, and Sandra Ceccatelli

6. Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures

Paul Honegger, Antoinette Defaux, Florianne Monnet-Tschudi, and Marie-Gabrielle Zurich

7. Neurospheres as a Model for Developmental Neurotoxicity Testing

Ellen Fritsche, Kathrin Gassmann, and Timm Schreiber

8. Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures

Pamela J. Lein, Christopher D. Barnhart, and Isaac N. Pessah

9. Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity

Mariaserena Boraso and Barbara Viviani

10. In Vitro Models to Study the Blood Brain Barrier

Hilary Vernon, Katherine Clark, and Joseph P. Bressler

Part II: Cell Death

11. Measurements of Cell Death in Neuronal and Glial Cells

Gennaro Giordano, Sungwoo Hong, Elaine M. Faustman, and Lucio G. Costa

12. Measurements of Neuronal Apoptosis

Gennaro Giordano and Lucio G. Costa

13. Measurement of Isoprostanes as Markers of Oxidative Stress

Dejan Milatovic, Thomas J. Montine, and Michael Aschner

14. Assessment of Glutathione Homeostasis

Gennaro Giordano, Collin C. White, and Lucio G. Costa

15. Assessing Neuronal Bioenergetic Status

Stephanie L.H. Zeiger, Jeannette N. Stankowski, and BethAnn McLaughlin

Part III: Signaling in Neurotoxicity

16. Fluorescent Assessment of Intracellular Calcium in Individual Cells

David P. Cox, Collin C. White, and Terrance J. Kavanagh

17. Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures

Victor Briz and Cristina Suñol

18. Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues

Ronald B. Tjalkens, David L. Carbone, and Guoyao Wu

19. JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons

Won-Seok Choi, Heather M Klintworth, and Zhengui Xia

20. Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration

Faneng Sun, Anumantha G. Kanthasamy, and Arthi Kanthasamy

21. Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo

Prasada Rao S. Kodavanti

22. Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons

Prasada Rao S. Kodavanti

Part IV: Other End-Points

23.


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Product Details
  • ISBN-13: 9781493958580
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: Softcover reprint of the original 1st ed. 2011
  • Language: English
  • Returnable: Y
  • Spine Width: 24 mm
  • Weight: 790 gr
  • ISBN-10: 1493958585
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 415
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm


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