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Laboratory Guide for the Bacteriologist: (English)

Laboratory Guide for the Bacteriologist: (English)

          
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About the Book

Purchase of this book includes free trial access to www.million-books.com where you can read more than a million books for free. This is an OCR edition with typos. Excerpt from book: 2. STAINING METHODS. The following are a few simple methods, in constant use, arranged with special attention to convenience in laboratory work1: I. STAINING BACTERIA IN COVER-GLASS PREPARATIONS WITH WATERY SOLUTIONS OF ANILINE DYES.' 1. MAKLNG THE COVER-GLASS PREPARATIONS. (a) From Fluid Parts of the Body.?With the sterilized straight or small looped platinum-needle place a small drop of blood or of pus upon the well-cleaned cover- glass and spread it out upon the latter in as even and as thin a film as possible; or, with a sterilized knife, a scraping from the freshly-cut surface of the organ to be examined is spread out in a like manner; or, the cover-glass is pressed directly against the freshly-cut surface of the organ. (Before cutting an organ burn the surface where the cut is to be made with the flat side of a very hot knife.) (b) From Pieces of Tissue.?With sterilized forceps pull out a small particle of tissue from the freshly-cut surface of the organ, crush between the forceps, and spread a portion of this crushed tissue substance (with the platinum-needle or the points of the forceps) upon the cover- glass as above directed. 2. FIXING THE COVER-GLASS PREPARATION. To fix the preparation the cover-slip is held in the forceps high abo-ve the flame, butter side uppermost, iFor anthrax, symptomatic anthrax (black-leg), hog cholera, mouse septicaemia, typhoid fever, gonococcus, tetanus, pus organisms, etc., and probably all saprophytic bacteria. 2 SOLUTIONS OF THE ANILINE DYES: (a) of the dye, 2 parts: water 85 parts; boil from five to ten minutes, and after cooling add 15 parts of 90 per cent, alcohol; mix thoroughly and filter twice, (b) Requisite amounts of concentrated alcoholic solutions of the dyes are diluted ten times with water. u...


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Product Details
  • ISBN-13: 9780217009409
  • Publisher: General Books
  • Publisher Imprint: General Books
  • Depth: 6
  • Language: English
  • Returnable: N
  • Spine Width: 2 mm
  • Width: 152 mm
  • ISBN-10: 0217009409
  • Publisher Date: 14 Oct 2010
  • Binding: Paperback
  • Height: 229 mm
  • No of Pages: 24
  • Series Title: English
  • Weight: 50 gr


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Laboratory Guide for the Bacteriologist: (English)
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