Recent advances in imaging technology reveal critical changes in living cells and organisms. This compendium of emerging techniques is organized into two parts: specific methods and experimental approaches.

Table of Contents:
Dedication Acknowledgments CD explanation Preface Foreword Contributors Introduction Section 1: Detection and Imaging Approaches 1. Fluorescent Protein Tracking and Detection M. Rizzo and D. Piston 2. Constructing and Expressing GFP-Fusion Proteins D. Spector and R. Goldman 3. Viral Vectors for Introduction of GPF into Living Cells R. Lansford 4. Gene Delivery by Direct Injection and Facilitation of Expression by Mechanical Stretch D. Dean 5. Microinjection of Fluorophore-labeled Proteins Y. Komarova, J. Peloquin, and G. Borisy 6. CCD Cameras for Fluorescence Imaging of Living Cells P. Tran 7. Photobleaching Techniques to Study Mobility and Molecular Dynamics of Proteins in Live Cells: FRAP, iFRAP, and FLIP R. Ellenberg and J. Ellenberg 8. FRET and Fluorescence Lifetime Imaging M. Tramier, D. Sanvitto, V. Emiliani, C. Durieux, and M. Coppey 9. Monitoring Protein Dynamics Using FRET-based Biosensors L. Chew and R. Chisholm 10. Application of Light-directed Activation of Caged Biomolecules and CALI (chromophore-assisted light inactivation) to Problems in Cell Motility D. Humphrey, Z. Rajfur, B. Imperiali, G. Marriott, P, Roy, and K. Jacobson 11. Photoactivation-based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus J. Politz, R. Ruft, and T. Pederson 12. Fluorescent Speckle Microscopy of Cytoskeletal Dynamics in Living Cells T. Wittman, R. Littlefield, and C. Waterman-Storrer 13. Polarization Microscopy with the LC-PolScope R. Oldenbourg 14. Confocal Microscopy, Deconvolution, and Structured Illumination Methods J. Murray 15. Multiphoton, Multispectral Laser Scanning Microscopy M. Dickinson 16. Analyzing Live Cell Data and Tracking Dynamic Movements W. Tvarusko, J. Mattes, and R. Eils Section 2: Imaging of live cells and organisms 17. In Vivo Imaging of Mammalian Cells J. Swedlow, P. Andrews, and M. Platani 18. A Sealed Preparation for Long-term Observation of Cultured Cells G. Sluder, J. Nordberg, F. Miller, and E. Hinchcliffe 19. Live Cell Imaging of Yeast D.R. Rines, D. Thomann, J.F. Dorn, P. Goodwin, and P.K. Sorger 20. Dynamic Imaging of C. elegans B. Podbilewicz and Y. Gruenbaum 21. Time-lapse Cinematography in Living Drosophila Tissues I. Davis and R.M. Parton 22. Single Cell Imaging in Animals J. Wyckoff, J. Segall, and J. Condeelis 23. Long-term, High-resolution Imaging in the Neocortex In Vivo B. Chen, J. Trachtenberg, A. Holtmaat, and K. Svoboda 24. Intravital Microscopy of Normal and Diseased Tissues in the Mouse R. Jain, E. Brown, L. Munn, and D. Fukumura 25. Development of Mammalian Cell Lines with Lac Operator Tagged Chromosomes Y. Strukov and A. Belmont 26. Imaging the Dynamics of Gene Expression D. Spector and S. Janicki 27. Studying Mitosis in Cultured Mammalian Cells P. Wadsworth 28. Imaging Hoechst 33342-labeled Chromosomes and Fluorescent Proteins During the Cell Cycle T. Haraguchi and Y. Hiraoka 29. Imaging the Actin Cytoskeleton A. Matus, V. Biou, H. Brinkhaus, and M. Roelandse 30. Dynamic Imaging of Intermediate Filament Systems R. Goldman 31. Monitoring the Motile Properties of Microtubules and Their Associated Proteins H. Goodson and P. Wadsworth 32. Imaging of Membrane Systems and Membrane Traffic in Living Cells J. Lippincott-Schwartz and E.L. Snapp 33. Imaging Live Cells Under Mechanical Stress B.P. Helmke and P.F. Davies 34. Imaging Single Molecules Using Total Internal Reflection Fluorescence Microscopy N. Stuurman and R.D. Vale 35. Visualization and Quantification of Single RNA Molecules in Living Cells Y. Shav-Tal, S.M. Shenoy, and R.H. Singer Video CD to accompany book Appendix 1: Suppliers Appendix 2: Cautions Index