John R. Chapman recreates the success of his earlier acclaimed book (Protein and Peptide Analysis by Mass Spectrometry, 1996) with a major new collection of cutting-edge methods in the analysis of proteins and peptides. Contributed by well-established investigators, each chapter provides background information before guiding the researcher step-by-step through the experimental process, with detailed instructions to help assure experimental success. The applications covered range widely and include protein sequencing, higher-order structure determination, epitope mapping, kinetics, quantitation, glycosylation analysis, and bacterial typing. Authoritative and eminently practical, Mass Spectrometry of Proteins and Peptides demonstrates the full scope of this versatile analytical technique. All protein chemists and biochemists, as well as bioanalytical scientists, who want to exploit these powerful methods in their work, will find this book indispensable.

Table of Contents:
De Novo Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry Using Triple Quadrupole and Quadrupole/Time-of-Flight Instruments Andrej Shevchenko, Igor Chernushevich, Matthias Wilm, and Matthias Mann Direct Analysis of Proteins in Mixtures: Application to Protein Complexes John R. Yates, III, Andrew J. Link, and David Schieltz Characterization of a Mutant Recombinant S100 Protein Using Electrospray Ionization Mass Spectrometry Mark J. Raftery Searching Sequence Databases via De Novo Peptide Sequencing by Tandem Mass Spectrometry Richard S. Johnson and J. Alex Taylor Signature Peptides: From Analytical Chemistry to Functional Genomics Haydar Karaoglu and Ian Humphery-Smith Investigating the Higher Order Structure of Proteins: Hydrogen Exchange, Proteolytic Fragmentation, and Mass Spectrometry John R. Engen and David L. Smith Probing Protein Surface Topology by Chemical Surface Labeling, Crosslinking, and Mass Spectrometry Keiryn L. Bennett, Thomas Matthiesen, and Peter Roepstorff Secondary Structure of Peptide Ions in the Gas Phase Evaluated by MIKE Spectrometry: Relevance to Native Conformations Igor A. Kaltashov, Aiqun Li, Zoltan Szilagyi, Karoly Vekey, and Catherine Fenselau Preparation and Mass Spectrometric Analysis of S-Nitrosohemoglobin Pasquale Ferranti, Gianfranco Mamone, and Antonio Malorni Multiple and Subsequent MALDI-MS On-Target Chemical Reactions for the Characterization of Disulfide Bonds and Primary Structures of Proteins H. Peter Happersberger, Marcus Bantscheff, Stefanie Barbirz, and Michael O. Glocker Epitope Mapping by a Combination of Epitope Excision and MALDI-MS Carol E. Parker and Kenneth B. Tomer Identification of Active Site Residues in Glycosidases by Use of Tandem Mass Spectrometry David J. Vocadlo and Stephen G. Withers Probing Protein Protein Interactions with Mass Spectrometry Richard W. Kriwacki and Gary Siuzdak Studies of Noncovalent Complexes in an Electrospray Ionization/Time-of-Flight Mass Spectrometer Andrew N. Krutchinsky, Ayeda Ayed, Lynda J. Donald, Werner Ens, Harry W. Duckworth, and Kenneth G. Standing Kinetic Analysis of Enzymatic and Nonenzymatic Degradation of Peptides by MALDI-TOFMS Fred Rosche, Jorn Schmidt, Torsten Hoffmann, Robert P. Pauly, Christopher H. S. McIntosh, Raymond A. Pederson, and Hans-Ulrich Demuth Characterization of Protein Glycosylation by MALDI-TOFMS Ekaterina Mirgorodskaya, Thomas N. Krogh, and Peter Roepstorff Positive and Negative Labeling of Human Proinsulin, Insulin and C-Peptide with Stable Isotopes: New Tools for In Vivo Pharmacokinetic and Metabolic Studies Reto Stocklin, Jean-Francois Arrighi, Khan Hoang-Van, Lan Vu, Fabrice Cerini, Nicolas Gilles, Roger Genet, Jan Markusssen, Robin E. Offord, and Keith Rose Identification of Snake Species by Toxin Mass Fingerprinting of Their Venoms Reto Stocklin, Dietrich Mebs, Jean-Claude Boulain, Pierre-Alain Panchaud, Henri Virelizier, and Cecile Gillard-Factor Mass Spectrometric Characterization of the b-Subunit of Human Chorionic Gonadotropin Roderick S. Black and Larry D. Bowers Analysis of Gluten in Foods by MALDI-TOFMS Enrique Mendez, Israel Valdes, and Emilio Camafeita Quantitation of Nucleotidyl Cyclase and Cyclic Nucleotide-Sensitive Protein Kinase Activities by Fast-Atom Bombardment Mass Spectrometry: A Paradigm for Multiple Component Monitoring in Enzyme Incubations by Quantitative Mass Spectrometry Russell P. Newton Influence of Salts, Buffers, Detergents, Solvents, and Matrices on MALDI-MS Protein Analysis in Complex Mixtures K. Olaf Bornsen Sample Preparation Techniques for Peptides and Proteins Analyzed by MALDI-MS Martin Kussmann and Peter Roepstorff Analysis of Hydrophobic Proteins and Peptides by Mass Spectrometry Johann Schaller Analysis of Proteins and Peptides Directly from Biological Fluids by Immunoprecipitation/Mass Spectrometry Sacha N. Uljon, Louis Mazzarelli, Brian T. Chait, and Rong Wang Detection of Molecular Determinants in Complex Biological Systems Using MALDI-TOF Affinity Mass Spectrometry Judy Van de Water and M. Eric Gershwin Rapid Identification of Bacteria Based on Spectral Patterns Using MALDI-TOFMS Jackson O. Lay, Jr. and Ricky D. Holland Appendices John R. Chapman