Part I: Investigation of Membrane Lipids
1. Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry
Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger
2. Thin-Layer Chromatography of Phospholipids
Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg
3. Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics
Martina Gsell and Günther Daum
Part II: Analysis of Structure and Topology of Membrane Proteins
4. Using 2D Crystals to Analyze the Structure of Membrane Proteins
Ian Collinson, Janet Vonck, and Dilem Hizlan
5. Crystallization of Membrane Proteins
Florian G. Müller and C. Roy D. Lancaster
6. Molecular Dynamics Simulations of Membrane Proteins
Kristyna Pluhackova, Tsjerk Wassenaar, and Rainer A. Böckmann
7. Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins
Christine T. Schwall and Nathan N. Alder
8. Topology Determination of Untagged Membrane Proteins
Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner
9. self-assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses
Katharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, and Maik S. Sommer
Part III: Studying Protein-Protein and Protein-Lipids Interactions within Membranes
10. The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane
Milit Marom and Abdussalam Azem
11. Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance
Zht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, and Arnold J.M Driessen
12. Peptide Interaction with and Insertion into Membranes
Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai
13. Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems
Joseph D. Unsay and Ana J. García-Sáez
14. Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast
Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo
15. Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins
Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, and Christine Ebel
16. Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry
Carolyn Vargas, Johannes Klingler, and Sandro Keller
Part IV: Membrane Biogenesis and Dynamics
17. Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria
Dirk Scholz, Johannes Förtsch, Stefan Böckler, Till Klecker, and Benedikt Westermann
18. Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells
Johanna Dudek, Sven Lang, Stefan Schorr, Johannes Linxweiler, Markus Greiner, and Richard Zimmermann
19. An Assay to Monitor the Membrane Integration of Single-Span Proteins
Katrin Krumpe and Doron Rapaport
20. Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria
Daniel Weckbecker and Johannes M. Herrmann
Part V: Functional Analysis of Membrane Proteins
21. Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes
Martin van der Laan, Ralf M. Zerbes, and Chris van der Does
22. Single Channel A
