About the Book
Disruption of mitochondrial energy production occurs commonly during cell injury and death. Efforts to understand mechanisms involved in toxic cell injury and to develop protective treatments therefore require a definition of the role of mitochondrial dysfunction. This volume approaches mitochondrial preparation and functional assessment from the perspective of toxic cell injury, with chapters contributed by experts representing a great diversity of biomedical disciplines. It is conveniently divided into six sections, addressing general methods, "in vivo" assessment, structure, detoxication and metabolic targets, bioenergetic and transport functions, and genetic analysis. The variety of approaches and subjects reflects the fundamental importance of mitochondria in aerobic cell survival. This volume should be of interest to researchers and students in toxicology, veterinary toxicology, toxicologic pathology, pharmacology and clinical biochemistry. It should provide a useful contemporary collection of papers critical to the research of all who are investigating mechanisms of cell injury.
Table of Contents:
Part 1: Systems for Study of Mitochondrial Dysfunc"don: L.H. Lash and J.M. Sail, NCtochondrial Isolation from Liver and Kidney: S trategy, Techniques, and Criteria for Purity. N-R . Sirm, Nfitochondrial Isolation from Brain: Strategy, Techniques, and Criteria for Purity. C. -P. Lee, M. Sciamanna, and P.L. Peterson, Intact Rat Brain Nfitochondria from a Single Animal: Preparation and Properties. M. Birch-Machin, S. Jackson, R.S. Kler, and D.M. Turnbull, Study of Skeletal Muscle Mtochondrial Dysfifnction. C. -P. Lee, M.E. Martens, and S.H. Tsang, Small-Scale Preparation of Skeletal Muscle NEtochondria and Its Application in the Study of Human Disease. H.V Westerhoff, B.H, Groen, and C. Van den Bogert, Applications of Metabolic Control Theory to Assessment of Mitochondrial Dysfunction. Part 2: Assessment of Mitochondrial Dysfunction in vivo and in Organ Systems: B. Chance, Commentary: Methods for in Vivo Assessment of Nfitochondrial Function. B.H. Lauterburg, Noninvasive Assess. ment of Nfitochondrial Function by Breath Analysis Using Ketoisocaproic Acid. CA. Piantadosi, Absorption Spectroscopy for in Vivo Assessment of Mitochondrial Dysfunction. C.R. MalloyA.D. Sherry, and FM. Jeffery, Analysis of Mtochondrial Function by 13C-NMR in Intact Tissues. M.R. Laughlin , 3 IP-NMR in the Study of Mitochondrial Metabolism. T Inoue andk. Tagawa, Mitochondrial Dysfunction in Ischemic Organs. R.J.M. Lane, Screening for Mitochondrial Cytopathy: The Subanaerobic Threshold Exercise Test. Part 3: Assay of Mitochondrial Structural Integrity: -S.R. Kayar, Morphometry of Mitochondria: Size,Internal Structure,Subcellular Distribution,and Three-Dimensional Reconstruction. C.L. Hoppel and B. Tandler, Megarnitochondria. K. Li-QUOc and D. Li-Qudc, Redox Statu4 and Mitochondrial Inner Membrane Permeability. Part 4: Mitochondrial Metabolism and Bioacdvatio@n and Detoxiflcation Systems: DJ. Reed, Assessment of Nfitochondrial Glutathione as a Measure of Cell Injury. X. Shan, M. Finkelstein, D.P. Jones, and M.W. Anders, Experimental Manipulation of NfitochondrialGlutathioneConcen@ons.RA. Harris, Y Zhao, and R. Paxton, Determination of Flux, Activity, and Activity State of Nfitochondrial a-Ketoacid Dehydrogenase Complexes. R.G. Thurman, Y Nakagawa, T Matsumara, JJ. Lemasters, and FC. Ka@n, Regulation of Oxygen Uptake in the Liver Lobule by Oxygen Tension. A.E. Vercesi and M.E. Ho@, Generation of Reactive Oxygen Metabolites and Oxidative Damage in Nttochondria: Role of Calcium. C.R. Roe, D.S. Roe, and D.S. Millington, Fatty Acid Metabolism and Reyes Syndrome. V Kagan and L. Packer, Antioxidative Function of Vitamin E and Ubiquinols. R.G. Thurman and FC. Kauffmn, NEtochondrial NADPH. J.B. McMillan, E.K. Hudson, and LM. Buja, Long-chain Acyl-CoA Metabolism by Mitochondrial Carnitine Palmitoyltranferase: A Cell Model for Pathological Studies. Part 5: Mitrochondrial Energetics and Transport Processes: W. Rouslin, Identification of Mitochondrial Dysfunction at Coupling Site I:
