Part I: Clinical Perspectives
1. Clinical Staging of Ovarian Cancer
Andrea S. O'Shea
2. Pathologic Classification of Ovarian Cancer
Stephanie M. McGregor
Part II: Tumor Microenvironment
3. Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer
Jessica Vazquez, Dagna Sheerar, Aleksandar K. Stanic, and Manish S. Patankar
4. Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors
Veronica D. Gonzalez, Ying-Wen Huang, and Wendy J. Fantl
5. Processing and Analysis of Ascites
Hannah M. Micek, Molly J. Carroll, Lisa Barroilhet, and Pamela K. Kreeger
6. Multispectral Staining and Analysis of Extracellular Matrix
Carine M. Renner, Mike R. Visetsouk, Pamela K. Kreeger, and Kristyn S. Masters
7. Quantitative Analysis of the Extracellular Matrix by Immunoblot
Kaitlin C. Fogg
Part III: Cell Isolation
8. Multiparameter Single Cell Characterization of Ovarian Intratumor Heterogeneity
Kristin G. Beaumont, Christina Andreou, Ethan Ellis, and Robert Sebra
9. Culturing Primary Human Mesothelial Cells
Mary Mullen, Hollie Noia, and Katherine Fuh
10. Isolation of Normal and Cancer-Associated Fibroblasts
Katarzyna Zawieracz and Mark A. Eckert
11. Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum
Abir Mukherjee
Part IV: Model Systems
12. Isolation of Fallopian Tube Epithelium and Ovaries for Assessment of Cilia Beating Frequency (CBF)
Angela Russo and Joanna E. Burdette
13. Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer
Matthew Dean
14. In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer
Elizabeth I. Harper and Tyvette S. Hilliard
15. The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance: 3D Co-Culture Methods
Michael Bregenzer, Eric Horst, Pooja Mehta, Catherine Snyder, Taylor Repetto, and Geeta Mehta
16. Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection
Sonam Mittal, Prachi Gupta, Pradeep Chaluvally-Raghavan, and Sunila Pradeep
17. Humanized Patient-Derived Xenograft Models of Ovarian Cancer
