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Practical Physiological Chemistry: (English)

Practical Physiological Chemistry: (English)

          
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About the Book

This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1914 Excerpt: ...(reduced alkaline haematin). Prepare a solution of alkaline haematin from dilute oxyhaemoglobin as in Ex. 217. Treat it with a few drops of ammonium sulphide. The colour of the solution changes to red. Examine with the spectroscope. Two absorption bands are seen in the green. The band nearer the D line (the a band) is very prominent and sharply defined, with its centre at about X 558. The /3 band is much fainter and has its centre at X 520. Note.--In very dilute solutions only the a band can be seen. The absorption of light in this region is so intense that if a solution of oxyhaemoglobin, so dilute that its absorption bands cannot be readily seen, be converted by appropriate means into haemochromogen, the a band of this pigment is usually observable. 220. Acid haematoporphyrin. To a few c.c. of concentrated sulphuric acid in a test tube add two drops of defibrinated blood (see note to Ex. 221) and mix by gentle shaking. Note the rich purple colour of the solution. Examine with the spectroscope. Two bands are seen: the a band, with centre at X 600, being fainter and narrower than the 5 band, centre X 554. 221. Alkaline haematoporphyrin. To the solution of acid haematoporphyrin just prepared add five or six more drops of defibrinated blood, shaking gently after the addition of each drop. Pour the strong solution into about 50 c.c. of cold water in a beaker, stir well and note the precipitate that rises to the surface. Transfer this precipitate to a test tube by means of a rod; treat it with a few c.c. of alcohol and boil. And 5 c.c. of sodium hydrate. A solution of alkaline haematoporphyrin is thus obtained, which examined spectroscopically after suitable dilutions shows a four banded spectrum. The centres of the bands are at X 622, X 576, X 539 and X 504 ap...


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Product Details
  • ISBN-13: 9781231119044
  • Publisher: Rarebooksclub.com
  • Publisher Imprint: Rarebooksclub.com
  • Height: 246 mm
  • No of Pages: 72
  • Series Title: English
  • Weight: 145 gr
  • ISBN-10: 1231119047
  • Publisher Date: 01 May 2012
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Spine Width: 4 mm
  • Width: 189 mm

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