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PRACTICAL PROBLEM SOLVING IN HPLC

PRACTICAL PROBLEM SOLVING IN HPLC

          
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About the Book

Table Of Contents

Introduction
SIMPLE TESTS AND DECISION CRITERIA
Tip 01: What is in a name of a column material?
Tip 02: Is this C18 column the right choice for my sample?
Tip 03: Why are polar solutes well separated with one C18 column and barely with another?
Tip 04: How can I clean the RP-phase fast?
Tip 05: How do I best degas my mobile phase?
Tip 06: Methanol or acetonitrile?
Tip 07: The pH-value of the mobile phase is too high/too low - what to do?
Tip 08: Which is the right ionic strength of the buffer?
Tip 09: How to make sense of the dead volume of an isocratic equipment?
Tip 10: Taking over a gradient method - the influence of the instrumentation
Tip 11: Does the pump work correctly, precisely or accurately? 
Tip 12: How to test an HPLC equipment and its modules?
Tip 13: Injection of solutes out of aqueous solutions
Tip 14: Which is the largest tolerable injection volume?
Tip 15: How critical are temperature changes? PartTip  I: General comments, Detector
Tip 16: How critical are temperature changes? Part TIP II: Column, Separation
Tip 17: How to choose an HPLC equipment and a supplier?
Tip 18: Is the current method a robust one? 
PROBLEMS AND THEIR SOLUTIONS
Tip 19: Sample preparation - how critical are which mistakes?
Tip 20: Flushing of an HPLC equipment
Tip 21: Junk in the UV detection cell
Tip 22: The lamp is new - what happened to the peak?
Tip 23: What are the causes for pressure changes or deviations?
Tip 24: Is the right or the left pump head defect?

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Product Details
  • ISBN-13: 9788126545223
  • Publisher Date: 2014
  • ISBN-10: 8126545224
  • Binding: Paperback

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