About the Book
1. Validation of Protein Acetylation by Mass Spectrometry
Barry M. Zee and Benjamin A. Garcia
2. Application of the CIRAD Mass Spectrometry Apprach for Lysine Acetylation Site Discovery
Caroline A Evans, Saw Yen Ow, Duncan L Smith, Bernard M Corfe, and Phillip C Wright
3. Application of the MIDAS Approach for Analysis of Lysine Acetylation Sites
Caroline A Evans, John R Griffiths, Richard D Unwin, Anthony D Whetton, Bernard M Corfe
4. Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein Acetylations
Bernard M Corfe, Josephine Kilner, Joanna Chowdry, Roderick SP Benson, Gareth J Griffiths, Caroline A Evans
5. Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin
Xumin Zhang and Peter Højrup
6. Identification and Analysis of O-acetylated Sialoglycoproteins
Chandan Mandal and Chitra Mandal
7. HPLC-based Quantification of in vitro N-terminal Acetylation
Rune H. Evjenth, Petra Van Damme, Kris Gevaert, Thomas Arnesen
8. Separation and Purification of Multiply Acetylated Proteins using Cation-exchange Chromatography
Romeo Papazyan and Sean D. Taverna
9. In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal Acetylation
Petra Van Damme, Thomas Arnesen, Bart Ruttens, Kris Gevaert
10. Computational Prediction of Lysine Acetylation Proteome-wide
Amrita Basu
11. Generation and Characterization of Pan-specific Anti-acetyllysine Antibody
Dr. Wei Xu and Dr. Shimin Zhao
12. Using Functional Proteome Microarrarys to Study Protein Lysine Acetylation
Jin-Ying Lu, Yu-yi Lin, Jef D. Boeke, and Heng Zhu
13. Quantitation of Nucleosome Acteylation and other Histone Post-Translational Using Microscale NU-ELISA
Bo Dai, Charles Giardina, Theodore P. Rasmussen
14. Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core
John C. Shimko, Cecil J. Howard, Michael G. Poirier, and Jennifer J. Ottesen
15. Production of Amino-terminally Acetylated Recombinant Proteins in E. coli
Matthew Johnson, Michael A. Geeves and Daniel P. Mulvihill
16. Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical Proteomics
Markus Grammel and Howard C. Hang
17. Non-radioactive in-vitro Assays for Histone Deacetylases
Alexander-Thomas Hauser, Julia M. Gajer (née Wagner), and Manfred Jung
18. The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes
Tielong Gao, Chao Yang, Yujun George Zheng
19. Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators
Mohammed Arif, Dhanasekaran Karthigeyan, Soumik Siddhanta, G. V. Pavan Kumar, Chandrabhas Narayana, and Tapas K. Kundu
