The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents:
Isolation, Synthesis, and Detection of DNA and RNA:F. Guidet and P. Langridge, Megabase DNA Preparation from Plant Tissue.M. Koob and W. Szybalski, Preparing and Using Agarose Microbeads.S. Anant and K. Subramanian, Methods for Rapid Isolation of Low Molecular Weight DNA from Bacteria and Animal Cells.J.D. Harding, R.L. Bebee, and G. Gebeyehu, Rapid, Specific DNA Isolation Using Methidium-Spermine-Sepharose.R.P. Kandpal, D.C. Ward, and S.M. Weissman, Chromosome Fishing: An Affinity Capture Method for Selective Enrichment of Large Genomic DNA Fragments.L. Ulanovsky, Trapping Electrophoresis of End-Modified DNA in Polyacrylamide Gels.C.C. Lee and C.T. Caskey, Direct cDNA Cloning Using PCR.Y.L. Chiang, Direct cDNA Cloning and Screening of Mutants Using the Polymerase Chain Reaction.J. Eberwine, C. Spencer, K. Miyashiro, S. Mackler, and R. Finnell, cDNA Synthesis in Situ: Methods and Applications.Z. Cai, J.K. Pullen, R.M. Horton, and L.R. Pease, Specific Amplification of cDNA from Targeted Members of Multigene Families.H. Toyoda, Long Synthetic Oligonucleotide Probes for Gene Analysis.D.J. Kemp, Colorimetric Detection of PCR-Amplified DNA Segments Using DNA Binding Proteins.A.M. Lew and M. Panaccio, A New Technique for Preparation of DNA from Blood for PCR in a Microtiter Tray.F.F. Chehab, J. Wall, and Y.W. Kan, Amplification and Detection of Specific DNA Sequences with FluorescentPCR Primers: Application to the ~gDF508 Mutation in Cystic Fibrosis.S. Beck, Nonradioactive Detection of DNA Using Dioxetane Chemiluminescence.H.C. Birnboim, Extraction of High Molecular Weight RNA and DNA from Cultured Mammalian Cells.C. Goblet, E. Prost, K.J. Bockhold, and R.G. Whalen, Comparison of One-Tube versus Two-Step Amplification of RNA Transcripts Using Polymerase Chain Reaction.A.D. Bharucha and M.R. Ven Murthy, Characterization of Polysomes and Polysomal mRNAs by Sucrose Density Gradient Centrifugation Followed by Immobilization in Polyacrylamide Gel Matrix.G. Levesque, A.D. Bharucha, and M.R. Ven Murthy, Biochemical Manipulations of Minute Quantitiesof mRNAs and cDNAs Immobilized on Cellulose Paper Discs.J. Soh and S. Pestka, Hybrid Selection of mRNA with Biotinylated DNA.Enzymes and Methods for Cleaving and Manipulating DNA:A.S. Bhagwat, Restriction Enzymes: Properties and Use.W.A. Bickmore and A.P. Bird, Use of Restriction Endonucleases to Detect and Isolate Genes from Mammalian Cells.D. Landry, J.M. Barsomian, G.R. Feehery, and G.G. Wilson, Characterization of Type II DNA Methyltransferases.G.G. Wilson, Amino Acid Sequence Arrangements of DNA Methyltransferases.M. Nelson and M. McClelland, Use of DNA Methyltransferase/Endonuclease Enzyme Combination for Megabase Mapping of Chromosomes.A. Podhajska, S.C. Kim, and W. Szybalski, Conferring New SPecificities on Restriction Enzymes: Cleavage at any Predetermined Site by Combining an Adapter Oligodeoxynucleotide and a Class-IIS Enzyme.S.A. Strobel and P.B. Dervan, Triple Helix Mediated Single-Site Enzymatic Cleavage of Megabase Genomic DNA.M. Koob, Creating New Cleavage Specificities of Restriction Endonucleases.C.W. Fuller, Modified T7 DNA Polymerase for DNA Sequencing.Reporter Genes:J. Gould, Nondesructive Assay for GUS in Culture Media of Plant Tissue Culture.B.R. Cullen and M.H. Malim, Secreted Placental Alkaline Phosphatase: A Novel Eukaryotic Reporter Gene.D.E. Hruby and E.M. Wilson, Use of a Fluorescent Chloramphenicol Derivative as a Substrate for CAT Assays.S. de la Luna and J. Ortin, Pac Gene as an Efficient Dominant Marker and Reporter Gene in Mammalian Cells.A.R. Brasier and D. Ron, Luciferase Reporter Gene Assay in Mammalian Cells.K.R. Luehrsen, J.R. de Wet, and V. Walbot, Transient Expression Analysis in Plants Using Firefly Luciferase Reporter Gene.K. D'Halluin, M. De Block, J. Denecke, J. Janssens, J. Leemans, A. Reynaerts, and J. Botterman, Bar Gene as Selectable and Screenable Marker in Plant Engineering.A. Caplan, R. Dekeyser, and M. Van Montagu, Useful Selectable Markers for Rice Transformation.M. Witty, Thaumatin II: A Sweet Marker Gene for Use in Plants.P.J. Punt and C.A.M.J.J. van den Hondel, Transformation of Filamentous Fungi Based on Hygromycin B and Phleomycin Resistance Markers.J. Altenbuchner, P. Viell, and I. Pelletier, New Positive Selection Vectors Based on Palindromic DNA Sequences.Vectors for Cloning Genes:J. Brosius, Compilation of Superlinker Vectors.M.A. Alting-Mees, J.A. Sorge, and J.M. Short, pBluescriptR II: Multifunctional Cloning and Mapping Vectors.J.M. Short and J.A. Sorge, In Vivo Excision Properties of Bacteriophage ~gl ZAPR Expression Vectors.I.-M.M. Chiu, K. Lehtoma, and M.L. Poulin, Cloning of cDNA Inserts from Phage DNA Directly into a Plasmid Vector.A.D. Zelenetz, Efficient Construction of Complex Directional cDNA Libraries in a Sfil Cassette.G.A. Evans, K. Snider, and G.G. Hermanson, Use of Cosmids and Arrayed Clone Libraries for Genome Analysis.J.C. Pierce and N.L. Sternberg, Using the Bacteriophase P1 System to Clone High Molecular Weight (HMW) Genomic DNA.K.D. Tartof, Cloning Vectors and Techniques for Exo-Hyb Restriction Mapping.R.H. Reeves, W.J. Pavan, and P. Hieter, YAC Modification and Manipulation.D.R. Smith, A.P. Smyth, and D.T. Moir, Copy Number Amplification of Yeast Artificial Chromosomes.R. Allshire, Manipulation of Large Minichromosomes in S. pombe with Liposome Enhanced Transformation.A.D. Miller, D.G. Miller, J.V. Garcia, and C.M. Lynch, Use of Retroviral Vectors for Gene Transfer and Expression.Author Index.Subject Index.