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Recombinant Gene Expression: Reviews and Protocols

Recombinant Gene Expression: Reviews and Protocols

          
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About the Book

Leading investigators, all of them experts on particular gene expression systems, provide a stellar set of original protocols for gene expression supported by overviews and troubleshooting guides for the biological systmes addressed. This book provides a global view of gene expression in prokaryotes, fungi, plants and animals and will be an invaluable reference in the collection of all biological science laboratories.

Table of Contents:
Table of Contents; Recombinant Gene Expression: Reviews and Protocols; Second Edition; Edited by Paulina Balbas and Argelia Lorence; Part 1. General Issues About Recombinant Gene Expression; 1 Host Cell Compatibility in Protein Expression James J. Greene; 2 Production of Recombinant Proteins: Challenges and Solutions Laura A. Palomares, Sandino Estrada Moncada, and Octavio T. Ramirez; 3 Folding Promoting Agents in Recombinant Protein Production: A Review Beatrix Fahnert; Part 2. Prokaryotes; 4 Back to Basics: pBR322 and Protein Expression Systems in E. coli. Paulina Balbas and Francisco Bolivar; 5 Alpha-Complementation Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments Mikhail F. Alexeyev, Viktoriya V. Pastukh, Inna N. Shokolenko, and Glenn L. Wilson; 6 Expression of Recombinant Alkaline Phosphatase Conjugates in E. coli Jean-Claude Boulain and Frederic Ducancel; 7 Overexpression of chromosomal genes in E. coli Fernando Valle and Noemi Flores; 8 Chromosomal Expression of Foreign and Native Genes from Regulatable Promoters in E. coli Lu Zhou, Ke Zhang and Barry L. Wanner; 9 Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in coli Sylvie Le Borgne, Francisco Bolivar, and Guillermo Gosset; 10 Copy-Control pBAC/oriV Vectors For Genomic Cloning Jadwiga Wild and Waclaw Szybalski; 11 Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV Jadwiga Wild and Waclaw Szybalski; 12 Cell-Free Protein Synthesis with Coupled Transcription and Translation James R. Swartz, Michael C. Jewett, and Kim A. Woodrow-Mumford; 13 Genetic Tools For The Manipulation of Moderately Halophilic Bacteria of The Family Halomonadaceae Carmen Vargas and Joaquin J. Nieto; 14 Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria Amalia S. Afendra, Carmen Vargas, Joaquin J. Nieto, and Constantin Drainas; 15 Recombinant Protein Production in Antarctic Gram-Negative Bacteria Angela Duilio, Maria Luisa Tutino, and Gennaro Marino; Part 3. Fungi; 16 Recombinant Protein Production in Yeasts Danilo Porro and Diethard Mattanovich; 17 Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae Kevin J. Vestrepen and Johan M. Thevelein; 18 High Throughput Expression in Microplate Formate in Saccharomyces cerevisiae Caterina Holz and Christine Lang; 19 High Throughput Expression in Microplate Formate in Pichia pastoris Mewes Botner and Christine Lang; 20 Multiple Gene Expression by Chromosomal Integration and CRE-loxP Mediated Marker Recycling in Saccharomyces cerevisiae Bjorn Johansson and Barbel Hahn-Hagerdal; 21 Fungal Transformation: Key Concepts and Applications Vianey Olmedo-Monfil, Carlos Cortes-Penagos, and Alfredo Herrera-Estrella; 22 Three Decades of Fungal Transformation: The Technologies Sergio Casas-Flores, Teresa Rosales-Saavedra, and Alfredo Herrera-Estrella; Part 4: Plants And Plant Cells; 23 Gene Transfer and Expression in Plants: A Review Argelia Lorence and Robert Verpoorte; 24 Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors Fabricio Medina-Bolivar and Carole Cramer; 25 Heterologous Gene Expression in Plant Chloroplasts Shashi Kumar and Henry Daniell


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Product Details
  • ISBN-13: 9781592597741
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 1592597742
  • Publisher Date: 14 May 2014
  • Binding: Digital
  • Sub Title: Reviews and Protocols


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