This detailed book aims to provide an overview of novel bioinformatics resources for exploring diverse aspects of RNA biology. This edition focuses on methods dealing with non-coding RNA (miRNAs, circRNAs or lncRNAs), RNA modifications (m6A or RNA editing), single cell RNA-seq and statistical models to handle count data from RNA-seq experiments. The book also includes chapters based on the classical RNA bioinformatics methods, such as those for deciphering secondary and tertiary RNA structures; however, they are revised to take into account deep sequencing data. Finally, chapters describing methods to analyze RNA sequencing data from emerging third generation sequencing technologies that could provide interesting insights into the transcriptional process and its regulation are also included.  Written for the highly successful Methods in Molecular Biology series, chapters include the kind of expert implementation advice that encourages quality results. Comprehensive and up-to-date, RNA Bioinformatics, Second Edition serves as an ideal guide for researchers digging ever-deeper into the depths of the study of RNAs. The chapter 'RNA-Seq Data Analysis in Galaxy' is open access under a CC BY 4.0 license.

Table of Contents:
Advanced Design of Structural RNAs Using RNARedPrint.- Modeling and Predicting RNA Three-Dimensional Structures.- Motif Discovery from CLIP Experiments.- Profiling of RNA Structure at Single-Nucleotide Resolution Using nextPARS.- RNA Framework for Assaying the Structure of RNAs by High-Throughput Sequencing.- Using RNentropy to Detect Significant Variation in Gene Expression across Multiple RNA-Seq or Single Cell RNA-Seq Samples.- Statistical Modeling of High Dimensional Counts.- QuickIsoSeq for Isoform Quantification in Large-Scale RNA Sequencing.- Summarizing RNA-Seq Data or Differentially Expressed Genes Using Gene Set, Network or Pathway Analysis.- Computational Analysis of circRNA Expression Data.- Differential Expression Analysis of Long Non-Coding RNAs.- Micro-RNA Quantification, Target Gene Identification, and Pathway Analysis.- In Silico Analysis of MicroRNA Sequencing Data.- RNA Editing Detection in HPC Infrastructures.- Identification of Genes Post-Transcriptionally Regulated from RNA-Seq: The Case Study of Liver Hepatocellular Carcinoma.- Computational Analysis of Single Cell RNA-Seq Data.- Normalization of Single-Cell RNA-Seq Data.- Dimensionality Reduction of Single-Cell RNA-Seq Data.- Single-Cell RNA Sequencing Analysis: A Step-by-Step Overview.- RNA-Seq Data Analysis in Galaxy.- RAP: A Web Tool for RNA-Seq Data Analysis.- iDEP Web Application for RNA-Seq Data Analysis.- Exploring Non-Invasive Biomarkers with the miRandola Database: A Tool for Translational Medicine.- Database Resources for Functional Circular RNAs.- Databases for RNA Editing Collections.- MODOMICS: An Operational Guide to the Use of the RNA Modification Pathways Database.- MeT-DB V2.0: Elucidating Context-Specific Functions of N6-Methyl-Adenosine Methyltranscriptome.- WHISTLE: A Functionally Annotated High-Accuracy Map of Human m6A Epitranscriptome.- Transcript Identification through Long-Read Sequencing.- Transcript Isoform-Specific Estimation of Poly(A)Tail Length by Nanopore Sequencing of Native RNA.- Nanopore RNA Sequencing Analysis.