The Isolated Hepatocyte: Use in Toxicology and Xenobiotic Biotransformations covers the link between research on the isolated hepatocyte and the disciplines of cell culture, toxicology, metabolism, and molecular biology. This book is composed of 11 chapters and begins with an overview of the regulation of liver growth, sulfation, glucuronidation of xenobiotics in specific liver sites. The next chapters deal with toxicology studies in cultured hepatocytes from various species and the in vitro control of hepatocyte proliferation. These topics are followed by discussions of choices and methods of cytotoxicity measures; hepatoxicity of metals; the metabolism and toxicity of xenobiotics in a primary culture; and the mechanism of carcinogen-induced pleiotropic drug resistance in hepatocytes. The concluding chapters describe the in vivo and in vitro measurement of chemically-induced DNA repair in hepatocytes, as well as the genotoxicity studies with human hepatocytes. This book is intended primarily to toxicologists and researchers.

Table of Contents:
Preface 1 Regulation of Liver Growth: Historical Perspectives and Future Directions I. Introduction II. Whole-Animal Studies III. Hepatocyte Cultures IV. Future Directions References 2 Regulation of Sulfation and Glucuronidation of Xenobiotics in Periportal and Pericentral Regions of the Liver Lobule I. Introduction II. Sulfation and Glucuronidation in Periportal and Pericentral Regions of the Liver Lobule III. Conclusion References 3 Toxicology Studies in Cultured Hepatocytes from Various Species I. Introduction II. Hepatocyte Primary Cultures: Methods III. Uses of Hepatocyte Primary Cultures for Xenobiotic Studies IV. Conclusions References 4 Cytochrome P-450-Dependent Monooxygenase Systems in Mouse Hepatocytes I. Introduction II. Methods Used to Study Drug Biotransformation in Isolated Hepatic Cells III. Applications IV. Conclusions References 5 Control of Hepatocyte Proliferation in Vitro I. Introduction II. Hepatocyte Isolation and Culture III. Epidermal Growth Factor IV. Norepinephrine V. Factors Derived from Serum or Platelets VI. Factors Derived from Normal Liver Cells VII. Factors Derived from Neoplastic Liver Cells References 6 Cytotoxicity Measures: Choices and Methods I. Introduction II. Plasma Membrane Integrity III. Subcellular Effects IV. Data Presentation V. In Vivo/in Vitro Correspondence VI. Methods References 7 Metals, Hepatocytes, and Toxicology I. Introduction II. Uptake and Efflux of Metals III. Use of Hepatocytes to Study Chelators IV. Toxicity of Metals V. Interactions among Metals VI. Mechanisms of Toxicity VII. Conclusion References 8 The Metabolism and Toxicity of Xenobiotics in a Primary Culture System of Postnatal Rat Hepatocytes I. Introduction II. Retention and Maintenance of Liver-Specific Differentiated Functions in Primary Culture of Postnatal Rat Hepatocytes III. Toxicity Evaluation of Xenobiotics in Cultured Hepatocytes IV. Metabolic Activation and Toxicity of Xenobiotics in Cultured Hepatocytes V. Conclusions References 9 The Analysis of Carcinogen-Induced Pleiotropic Drug Resistance in Rat Hepatocytes I. Introduction II. Study of Drug Resistance in Carcinogen-Altered Hepatocytes III. Cytotoxicity Studies in Vitro IV. Cellular Pharmacokinetics of Adriamycin in Sensitive and Resistant Hepatocytes V. Membrane Actions of Adriamycin VI. Conclusions References 10 Measurement of Chemically Induced DNA Repair in Hepatocytes in Vivo and in Vitro as an Indicator of Carcinogenic Potential I. Introduction II. DNA Repair III. Procedures for in Vitro and in Vivo Rat Hepatocyte DNA Repair Assays References 11 Genotoxicity Studies with Human Hepatocytes I. Introduction II. Isolation and Culture of Human Hepatocytes III. Hepatocyte Cell Culture IV. Cryopreservation of Human Hepatocytes V. Genotoxicity Studies with Human Hepatocytes VI. Xenobiotic Metabolism by Human Hepatocytes VII. Conclusions References Index