Whether considering toxicant exposure in zebrafish, or the application of cellular diagnostics to marine toxicology, or the ecotoxicology of coral reef ecosystems, or the amount of metalloids in water, this reference offers the protocols for specimen collection that researchers need. Following up on his popular Techniques in Aquatic Toxicology with a second volume, now nine years later, Dr. Ostrander has once again called on the top aquatic toxicologists from across the world to present 39 chapters of unique collection and testing procedures.

Updating five techniques from the first volume, the authors have gone on to add over two dozen new techniques. Like the first volume, this text divides the chapters into four broad areas: Techniques for the Assessment of Toxicity in Whole Organisms, Cellular and Subcellular Toxicity, Contaminant Identification, and Impacts in Aquatic Ecosystems, and ends with a General Techniques section that anyone working in the field should find useful.

Every chapter covers a specific procedure that can easily be reproduced by any competent technician with basic knowledge. Each of the chapter authors provides and interprets typical as well as anomalous results, false positives, and artifacts. Data is provided either from recently published experiments or from work being published for the first time.

Table of Contents:
Techniques for assessment of toxicity in whole organisms
Integrative measures of toxicant exposure in zebrafish (Danio rerio) at different levels of biological organization. Roel Smolders, Wim De Coen, and Ronny Blust

Use of disease challenge assay to assess immunotoxicity of xenobiotics in fish. Mary R. Arkoosh, Deborah Boylen, Coral L. Stafford, Lyndal L. Johnson, and Tracy K. Collier

Enhanced frog embryo teratogenesis assay - Xenopus (FETAX) model using Xenopus tropicalis.Douglas J. Fort and Robert L. Rogers

A short-term mummichog (Fundulus heteroclitus) bioassay to assess endocrine responses to hormone-active compounds and mixtures. Deborah L. MacLatchy, Karen L. Gormley, Rebecca E. M. Ibey, Rainie L. Sharpe, Kevin S. Shaughnessy, Simon C. Courtenay, Monique G. Dubé, and Glen J. Van Der Kraak

Conducting dose-response feeding studies with salmonids: growth as an endpoint. James P. Meador, Frank C. Sommers, Robert J. Wolotira, and Leslie Kubin

Field experiments with caged bivalves to assess chronic exposure and toxicity. Michael H. Salazar and Sandra M. Salazar

Application of computer microscopy for histopathology in isopod toxicity studies. Damjana Drobne and Samo Drobne

Sperm cell and embryo toxicity tests using the sea urchin Paracentrotus lividus (LmK). Annamaria Volpi Ghirardini, Alessandra Arizzi Novelli, Chiara Losso, and Pier Francesco Ghetti

Assessment of metal toxicity to sulfate reducing bacteria through metal concentration methods. Vivek P. Utgikar, and Henry H. Tabak

Techniques for measurement of cellular and subcellular toxicity
Cellular diagnostics and its application to aquatic and marine toxicology. Craig A. Downs

A non-destructive technique to measure cytochrome P4501A enzyme activity in living embryos of the estuarine fish Fundulus heteroclitus. Diane Nacci, Laura Coiro, Deena M. Wassenberg, and Richard T. Di Giulio

Determination of lipid classes and lipid content in tissues of aquatic organisms using a thin layer chromatography/flame ionization detection (TLC/FID) microlipid method. Gina M. Ylitalo, Gladys K. Yanagida, Lawrence Hufnagle, Jr., and Margaret M. Krahn

Larval molting hormone synthesis and imaginal disc development in the midge Chironomus riparius as tools for assessing the endocrine modulating potential of chemicals in aquatic insects. Torsten Hahn

The electro-olfactogram: an in vivo measure of peripheral olfactory function and sublethal neurotoxicity in fish. David H. Baldwin and Nathaniel L. Scholz

Enzyme-linked immunosorbent assay for screening estrogen receptor binding activity
Tomoko Koda, Yoshihiro Soya, and Masatoshi Morita

Lysosomal destabilization assays for estuarine organisms. A. H. Ringwood, D. E. Conners, J. Hoguet, and L. A. Ringwood

IMCOMP-P: an assay for coral immuno-competence. Craig A. Downs, Aaron G. Downs, Robert B. Jonas, Kay Briggs, Thomas Capo, and Cheryl M. Woodley

Monitoring gene expression in Rana catesbeiana tadpoles using a tail fin biopsy technique and its application to the detection of environmental endocrine disruptor effects in wildlife species. Nik Veldhoen and Caren C. Helbing

Techniques for identification and assessment of contaminants in aquatic ecosystems
Coral reproduction and recruitment as tools for studying the ecotoxicology of coral reef ecosystems. Robert H. Richmond

Using the stickleback to monitor androgens and anti-androgens in the aquatic environment. Ioanna Katsiadaki

Simple methods for estimating exposure concentrations of pesticide resulting from non-point source applications in agricultural drainage networks. Wenlin Chen

Design and analysis of toxicity tests for the development and validation of biotic ligand models for predicting metal bioavailability and toxicity. Karel A.C. DeSchamphelaere, Dagobert G. Heijerick, and Colin R. Janssen

Rapid toxicity fingerprinting of polluted waters using lux-marked bacteria. Nigel L. Turner

Aquatic in situ bioassays to detect agricultural non-point source pesticide pollution: a link between laboratory and field. Ralf Schulz

Improvements to high-performance liquid chromatography/photodiode array detection (HPLC/PDA) method that measures dioxin-like polychlorinated biphenyls and other selected organochlorines in marine biota. Gina M. Ylitalo, Jon Buzitis, Daryle Boyd, David P. Herman, Karen L. Tilbury, and Margaret M. Krahn

Estrogenic activity measurement in wastewater using in vitro and in vivo methods. Yelena Sapozhnikova, Anne McElroy, Shane Snyder, and Daniel Schlenk

A toxicity assessment approach for evaluation of in-situ bioremediation of PAH contaminated sediments. Henry H. Tabak, James M. Lazorchak, Mark E. Smith, and Jim Ferretti

Application of solid-phase microextraction fibres as biomimetic sampling devices in ecotoxicology. Roman Lanno, James M. Conder, Jason B. Wells, and Thomas W. La Point

Passive dosemeters for measurement of ultraviolet radiation in aquatic environments. C.S Sinclair, R.H. Richmond, E.T. Knobbe, C. Basslear, and G.K. Ostrander

Techniques for aquatic toxicologists
Spectral models for assessing exposure of fish to contaminants. Donald C. Malins, John J. Stegeman, Virginia M. Green, Naomi K. Gilman, and Katie M. Anderson

Design and use of a highly responsive and rigidly controllable hypoxia exposure system. D. W. Lehmann, J. F. Levine, and J. M. Law

Fish models in behavioral toxicology: automated techniques, updates and perspectives. Andrew S. Kane, James D. Salierno, and Sandra K. Brewer

Measuring metals and metalloids in water, sediment, and biological tissues. Michael C. Newman and Yuan Zhao

Estimation of inorganic species aquatic toxicity. James P. Hickey

Determining aromatic hydrocarbons and chlorinated hydrocarbons in sediments and tissues using accelerated solvent extraction and gas chromatography/mass spectrometry. C.A. Sloan, D.W. Brown, R.W. Pearce, R.H. Boyer, J.L. Bolton, D.G. Burrows, D.P. Herman, and M.M. Krahn

Histological preparation of invertebrates for evaluating contaminant effects. Esther C. Peters, Kathy L. Price, and Doranne J. Borsay Horowitz

Isolation of genes in aquatic animals using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. Jeanette M. Rotchell

Analysis of mutations in ? transgenic medaka using the cll mutation assay. Richard N. Winn and Michelle B. Norris

Improved methods of conducting microalgal bioassays using flow cytometry. M. Franklin, Jennifer L. Stauber, and Merrin S. Adams