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Home > Tecniques de Laboratori: PCR En Temps Real, Reaccio En Cadena de La Polimerasa, Electroforesi En Gel D'Agarosa, Sequenciacio D'Adn(Catalan)
Tecniques de Laboratori: PCR En Temps Real, Reaccio En Cadena de La Polimerasa, Electroforesi En Gel D'Agarosa, Sequenciacio D'Adn(Catalan)

Tecniques de Laboratori: PCR En Temps Real, Reaccio En Cadena de La Polimerasa, Electroforesi En Gel D'Agarosa, Sequenciacio D'Adn(Catalan)

          
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About the Book

Font: Wikipedia. Pagines: 37. Capitols: PCR en temps real, Reaccio en cadena de la polimerasa, Electroforesi en gel d'agarosa, Sequenciacio d'ADN, Destil.lacio, Series de Balmer, Centrifugacio diferencial, Triangle de Perkin, Polimorfisme de longitud del fragment de restriccio, Medi de creixement, Metode de l'ADN ramificat, Cultiu tissular de plantes, SDS-PAGE, Cultiu cel.lular, Ressonancia magnetica nuclear, Orbitrap, Nefelometria, Filtracio, Extraccio, Polarimetria, Enginyeria del buit, Eosina, Index de peroxid, Cultiu microbiologic, Marcatge per afinitat amb codificacio isotopica, Isoelectroenfocament, ELISA, Destil.lacio seca, Transferencia northern, Bany maria, Transferencia Southern, Turbidimetria, Atmolisi. Extracte: La PCR quantitativa, qPCR, Q-PCR o PCR en temps real es una variant de la reaccio en cadena de la polimerasa (PCR) que es fa servir per amplificar i quantificar en termes absoluts el producte de l'amplificacio, l'acid desoxiribonucleic. Igual que en la PCR convencional, s'empra un motlle d'ADN, almenys un parell d'encebadors especifics, dNTP, un amortidor de reaccio adequat i una polimerasa d'ADN termoestable. S'afegeix a aquesta barreja una substancia marcada amb un fluorofor que, en un termociclador dotat de sensors per mesurar fluorescencia, despres de ser excitat a la longitud d'ona apropiada, permeti mesurar la taxa de generacio d'un o mes productes especifics. Aquest mesurament es fa despres de cada cicle d'amplificacio, motiu pel qual tambe se la denomina PCR en temps real. En molts casos el motlle emprat per la PCR quantitativa no es ADN des del principi, sino que pot ser ADN complementari (ADNc) monocatenari, obtingut per retrotranscripcio de l'acid ribonucleic (ARN); en aquest cas, s'utilitzen les denominacions RT-PCR quantitativa, RT-PCR en temps real o RT-Q-PCR. No s'ha de confondre amb la tecnica de la PCR despres de transcripcio inversa en que hi ha un pas de retrotranscripcio d'ARN a ADN que no quantifica necessariament el prod...


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Product Details
  • ISBN-13: 9781232737513
  • Publisher: Books LLC, Wiki Series
  • Publisher Imprint: Books LLC, Wiki Series
  • Height: 246 mm
  • No of Pages: 38
  • Series Title: Catalan
  • Sub Title: PCR En Temps Real, Reaccio En Cadena de La Polimerasa, Electroforesi En Gel D'Agarosa, Sequenciacio D'Adn
  • Width: 189 mm
  • ISBN-10: 1232737518
  • Publisher Date: 04 Aug 2011
  • Binding: Paperback
  • Language: Catalan
  • Returnable: N
  • Spine Width: 2 mm
  • Weight: 86 gr


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