Home > Mathematics and Science Textbooks > Biology, life sciences > Biochemistry > Two-Hybrid Systems: Methods and Protocols(1794 Methods in Molecular Biology)
37%
Two-Hybrid Systems: Methods and Protocols(1794 Methods in Molecular Biology)

Two-Hybrid Systems: Methods and Protocols(1794 Methods in Molecular Biology)

          
5
4
3
2
1

Available


Premium quality
Premium quality
Bookswagon upholds the quality by delivering untarnished books. Quality, services and satisfaction are everything for us!
Easy Return
Easy return
Not satisfied with this product! Keep it in original condition and packaging to avail easy return policy.
Certified product
Certified product
First impression is the last impression! Address the book’s certification page, ISBN, publisher’s name, copyright page and print quality.
Secure Checkout
Secure checkout
Security at its finest! Login, browse, purchase and pay, every step is safe and secured.
Money back guarantee
Money-back guarantee:
It’s all about customers! For any kind of bad experience with the product, get your actual amount back after returning the product.
On time delivery
On-time delivery
At your doorstep on time! Get this book delivered without any delay.
Quantity:
Add to Wishlist

About the Book

This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions.  The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include thekind of detail and key implementation advice that leads to excellent results in the lab.  Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.

Table of Contents:
Protein Interactomics by Two-Hybrid Methods.- Making the Right Choice: Critical Parameters of the Y2H Systems.- Identification of Protein-Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening.- Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System.- The Ras Recruitment System (RRS) for the Identification and Characterization of Protein-Protein Interactions.- A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning.- Analyses of Protein Interaction Networks Using Computational Tools.- Options and Considerations When Using a Yeast One-Hybrid System.- Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins.- Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens.- Transcription Factor-Centered Yeast One-Hybrid Assay.- The Yeast Three-Hybrid System for Protein Interactions.- The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants.- Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules.- Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System.- Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells.- Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Human Cells Based on Gateway Cloning Technology.- KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein-Protein Interactions.- Coimmunoprecipitation of Interacting Proteins in Plants.- Analysis of Protein-Protein Interaction by Co-IP in Human Cells.- Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures.- Transient Transactivation Studies in Nicotiana benthamiana Leaves.- The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions.- Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells.


Best Sellers


Product Details
  • ISBN-13: 9781493978700
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: 1st ed. 2018
  • Language: English
  • Returnable: Y
  • Spine Width: 24 mm
  • Weight: 7612 gr
  • ISBN-10: 1493978705
  • Publisher Date: 01 Jun 2018
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 355
  • Series Title: 1794 Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm


Similar Products

How would you rate your experience shopping for books on Bookswagon?

Add Photo
Add Photo

Customer Reviews

REVIEWS           
Click Here To Be The First to Review this Product
Two-Hybrid Systems: Methods and Protocols(1794 Methods in Molecular Biology)
Humana Press Inc. -
Two-Hybrid Systems: Methods and Protocols(1794 Methods in Molecular Biology)
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Two-Hybrid Systems: Methods and Protocols(1794 Methods in Molecular Biology)

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book
    Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Bookswagon (the "CRR Service").


    By submitting any content to Bookswagon, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Bookswagon (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Bookswagon a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Bookswagon may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Bookswagon's sole discretion. Bookswagon reserves the right to change, condense, withhold publication, remove or delete any content on Bookswagon's website that Bookswagon deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Bookswagon does not guarantee that you will have any recourse through Bookswagon to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Bookswagon reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Bookswagon, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Bookswagon, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals


    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!
    ASK VIDYA