Part I: Background and Overview
1. Enabling Molecular Technologies for Trait Improvement in Wheat
Prem L. Bhalla, Akanksha Sharma, and Mohan B. Singh
2. What Will Be the Benefits of Biotech Wheat for European Agriculture?
Agnès E. Ricroch
3. Overview of the Wheat Genetic Transformation and Breeding Status in China
Jiapeng Han, Xiaofen Yu, Junli Chang, Guangxiao Yang, and Guangyuan He
4. Wheat Improvement in India: Present and Future
Sonia Goel, Kalpana Singh, and N.K. Singh
5. Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines
Rohit Joshi, Khalid Anwar, Priyanka Das, Sneh L. Singla-Pareek, and Ashwani Pareek
6. Allergenicity Assessment of Transgenic Wheat Lines In Silico
Ankita Mishra and Naveen Arora
7. Agribusiness Perspectives on Transgenic Wheat
Bill Malcolm
Part II: Protocols
8. Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos
Goetz Hensel, Cornelia Marthe, and Jochen Kumlehn
9. Biolistic Transformation of Wheat
Caroline Tassy and Pierre Barret
10. Wheat Genetic Transformation Using Mature Embryos as Explants
Harsh Chauhan and Paramjit Khurana
11. Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems
Yanpeng Wang, Yuan Zong, and Caixia Gao
12. Design and Assembly of CRISPR/Cas9 Reagents for Gene Knock-Out, Targeted Insertion, and Replacement in Wheat
Tomás Čermák and Shaun J. Curtin
13. Doubled Haploid Transgenic Wheat Lines by Microspore Transformation
Sachin Rustgi, Nii O. Ankrah, Rhoda A.T. Brew-Appiah, Yue Sun, Weiguo Liu, and Diter von Wettstein
14. Doubled Haploid Laboratory Protocol for Wheat Using Wheat-Maize Wide Hybridization
Meenakshi Santra, Hong Wang, Scott Seifert, and Scott Haley
15. Real-Time PCR for the Detection of Precise Transgene Copy Number in Wheat
Angelica Giancaspro, Agata Gadaleta, and Antonio Blanco
16. Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection
Litao Yang, Sheng Quan, and Dabing Zhang
17. Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum
