Part I: Introduction to Yeast Surface Display and Its Applications
1. Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System
Maryam Raeeszadeh-Sarmazdeh and Eric T. Boder
Part II: Construction and Selection of Yeast Surface Display Libraries and Analysis of Isolated Variants
2. Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation
Byong H. Kang, Brianna M. Lax, and K. Dane Wittrup
3. Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations
Benedikt Dolgikh and Daniel Woldring
4. Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution
James VanAntwerp, Patrick Finneran, Benedikt Dolgikh, and Daniel Woldring
5. Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries
Mehrsa Mardikoraem and Daniel Woldring
6. Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders
Nicole J. Yang
7. Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display
Preeti Sharma, Erik Procko, and David M. Kranz
8. Engineering Binders with Exceptional Selectivity
Kai Wen Teng, Akiko Koide, and Shohei Koide
9. Affinity and Stability Analysis of Yeast Displayed Proteins
Charlotte U. Zajc, Magdalena Teufl, and Michael W. Traxlmayr
Part III: Selection of Yeast Surface Display Libraries for Binding to Mammalian Cells or Extracellular Matrix
10. Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting
Stefania C. Carrara, Jan P. Bogen, Julius Grzeschik, Björn Hock, and Harald Kolmar
11. A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins
Patrick J. Krohl and Jamie B. Spangler
12. Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques
Rojhae A. Panton and Lawrence A. Stern
13. Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display
Benjamin J. Umlauf, John S. Kuo, and Eric V. Shusta
Part IV: Specialized Yeast Surface Display Applications
14. Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology
Sara Linciano, Ee Lin Wong, Ylenia Mazzocato, Monica Chinellato, Tiziano
