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Hepatitis B and D Protocols: Volume 1: Detection, Genotypes, and Characterization(95 Methods in Molecular Medicine)

Hepatitis B and D Protocols: Volume 1: Detection, Genotypes, and Characterization(95 Methods in Molecular Medicine)

          
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About the Book

For volume 1: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. For both volumes: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. The first volume Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. The second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. The set offers both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, an essential resource for finding new therapies to treat chronically infected patients.

Table of Contents:
Detection of Viral Markers.- Overview of Commercial HBV Assay Systems.- Detection of HBV DNA in Serum Using a PCR-Based Assay.- Detection of HBV DNA by Oligonucleotide Probing.- Detection of HBV RNA in Serum of Patients.- Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR.- In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sections.- Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresis.- In Situ Detection of Hepatitis B Viral Antigens.- Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting.- Detection of Serum HDV RNA by RT-PCR.- Nonradioisotopic In Situ Hybridization for HDV RNA.- Detection and Characterization of Small and Large HDV Antigens.- Immunohistochemical Detection of Hepatitis Delta Antigen.- Viral Genotypes and Variants.- HBV Vaccine-Escape Variants.- HBV Genotyping and Analysis for Unique Mutations.- A One-Filter–Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus.- Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletion.- Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assay.- Molecular Biological Characterization.- Processing of Hepatitis B Virus Surface Proteins.- Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus.- Nucleoprotein Transport of HBV Capsid Particles.- Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian Cells.- Study of HBV Replicaton Capcity in Relation to Sequence Variation in the Precore and Core Promoter Regions.- A cis/trans Genetic Test for PleiotropicPhenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein.- Studying DHBV Polymerase by In Vitro Transcription and Translation.- Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell System.- Localization of Duck Hepatitis B Virus Polymerase Within Cells.- Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Mice.- Transcriptional Control of Hepatitis B Virus.- In Vitro Reconstitution of ?-Dependent Duck Hepatitis B Virus Replication Initiation.- Hepatitis B Viruses.


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Product Details
  • ISBN-13: 9781588291059
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Edition: 2004 ed.
  • Language: English
  • Returnable: N
  • Spine Width: 25 mm
  • Weight: 725 gr
  • ISBN-10: 1588291057
  • Publisher Date: 28 Jan 2004
  • Binding: Hardback
  • Height: 229 mm
  • No of Pages: 376
  • Series Title: 95 Methods in Molecular Medicine
  • Sub Title: Volume 1: Detection, Genotypes, and Characterization
  • Width: 152 mm


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