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Hepatitis B and D Protocols: v.1 Detection,Genotypes,and Characterization

Hepatitis B and D Protocols: v.1 Detection,Genotypes,and Characterization

          
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About the Book

For volume 1: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. For both volumes:

Table of Contents:
Part I. Detection of Viral Markers; Overview of Commercial HBV Assay Systems Stefan Zeuzem; Detection of HBV DNA in Serum Using a PCR-Based Assay Hau Tim Chung; Detection of HBV DNA by Oligonucleotide Probing Hsiang Ju Lin; Detection of HBV RNA in Serum of Patients Wei Zhang, Hans Jorg Hacker, Maria Mildenberger, Qin Su, and Claus H. Schroder; Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR Scott Bowden, Kathy Jackson, Margaret Littlejohn, and Stephen Locarnini; In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sections Vicky C. H. Lai and Johnson Y. N. Lau; Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresis Wolfram H. Gerlich, Ulrike Wend, and Dieter Glebe; In Situ Detection of Hepatitis B Viral Antigens: An Immunohistochemical Approach Jane W. S. Fang and Johnson Y. N. Lau; Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting Jie Liu and Mark A. Feitelson; Detection of Serum HDV-RNA by RT-PCR Antonina Smedile, Maria Grazia Niro, and Mario Rizzetto; Nonradioisotopic In Situ Hybridization for HDV RNA Francesco Negro; Detection and Characterization of Small and Large HDV Antigens Thomas B. Macnaughton and Michael M. C. Lai; Immunohistochemical Detection of Hepatitis Delta Antigen Karim Abid and Francesco Negro; Part II. Viral Genotypes and Variants; HBV Vaccine-Escape Variants Ashraf A. Basuni and William F. Carman; HBV Genotyping and Analysis for Unique Mutations Anna Ayres, Stephan Locarnini, and Angeline Bartholomeusz; A One-Filter-Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus Chiaho Shih and Ta-Tung Thomas Yuan; Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletion Chiaho Shih and Pei-Ching Tai; Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assay Sija De Gendt, Fred Shapiro, Jelena Juras, Els Van Assche, Geert Maertens, and Erwin Sablon; Part III. Molecular Biological Characterization; Processing of Hepatitis B Virus Surface Proteins Volker Bruss; Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus Stephan Urban; Nucleoprotein Transport of HBV Capsid Particles Michael Kann; Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian cells Jie Li, Yanyan Zheng, Jinah Choi, and Jing-hsiung Ou; Study of HBV Replication Capacity in Relation to Sequence Variation in the Precore and Core Promoter Regions Fabien Zoulim, ShuPing Tong, and Christian Trepo; A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein Chiaho Shih and Ta-Tung Thomas Yuan; Studying DHBV Polymerase by In Vitro Transcription and Translation Jianming Hu; Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell System Lisa Lott, Lena Notvall, and Robert E. Lanford; Localization of Duck Hepatitis B Virus Polymerase Within Cells Ermei Yao and John E. Tavis; Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Mice Zhenming Xu and Jing-hsiung Ou; Transcriptional Control of Hepatitis B Virus Anneke K. Raney; In Vitro Reconstitution of [epsilon]-Dependent Duck Hepatitis B Virus Replication Initiation


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Product Details
  • ISBN-13: 9781592596690
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Language: English
  • ISBN-10: 159259669X
  • Publisher Date: 14 May 2014
  • Binding: Digital
  • Sub Title: v.1 Detection,Genotypes,and Characterization


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