Laboratory Applications in Microbiology: A Case Study Approach includes a photo atlas with more than 250 full-color images! This lab uses real-life case studies as the basis for exercises in the laboratory. This is the only microbiology lab manual focusing on this means of instruction, an approach particularly applicable to the microbiology laboratory. The author has carefully organized the exercises so that students develop a solid intellectual base beginning with a particular technique, moving through the case study, and finally applying new knowledge to unique situations beyond the case study.

Table of Contents:
Case Study Exercise 1 Safety Considerations in theMicrobiology Laboratory Case Study Exercise 2 Microscopy and Measurement of Microscopic SpecimensCase Study Exercise 3 Identification and Classification of Algae Case Study Exercise 4 Survey of Medically ImportantProtozoa Case Study Exercise 5 Identification andClassification of Fungi Case Study Exercise 6 Ubiquity ofMicroorganisms Case Study Exercise 7 Aseptic and Pure Culture TechniquesCase Study Exercise 8 Simple Staining, Negative Staining, and Gram StainingCase Study Exercise 9 Endospore StainingCase Study Exercise 10 Acid-Fast and Endospore StainingCase Study Exercise 11 Acid-Fast StainingCase Study Exercise 12 Viable Plate Count Case Study Exercise 13 Cultivation of AnaerobesCase Study Exercise 14 Temperature Effects onBacterial Growth and SurvivalCase Study Exercise 15 pH and Microbial GrowthCase Study Exercise 16 Effects of Osmotic Pressureon Bacterial GrowthCase Study Exercise 17 Lethal Effects of UltravioletLightCase Study Exercise 18 Evaluation of DisinfectantsCase Study Exercise 19 Effectiveness of HandScrubbingCase Study Exercise 20 Antimicrobic SensitivityTesting: Kirby-Bauer, Tube Dilution, and E-TestMethodsCase Study Exercise 21 Phage Typing of BacteriaCase Study Exercise 22 Simulated EpidemicCase Study Exercise 23 Morbidity and MortalityWeekly ReportCase Study Exercise 24 Bacterial TransformationCase Study Exercise 25 The Ames TestCase Study Exercise 26 DNA Extraction fromBacterial CellsCase Study Exercise 27 DNA ProfilingCase Study Exercise 28 Measures of Water Quality:Most Probable Number ProcedureCase Study Exercise 29 Measures of Water Quality:Membrane Filtration MethodCase Study Exercise 30 Measures of Milk Quality:Methylene Blue Reductase TestCase Study Exercise 31 Bacterial Counts of FoodCase Study Exercise 32 Isolation and Identification of StaphylococciCase Study Exercise 33 Isolation and Identification of StreptococciCase Study Exercise 34 Epidemiology of Gastrointestinal Illness: Differentiation of EnterobacteriaceaeCase Study Exercise 35 Blood TypingCase Study Exercise 36 Differential White BloodCase Study Exercise 37 Slide AgglutinationCase Study Exercise 38 Enzyme-LinkedImmunosorbent Assay (ELISA)Case Study Exercise 39 Identification of BacterialUnknownsExercise 40 Colony MorphologyExercise 41 Growth in Solid and Liquid MediaExercise 42 Motilty Methods: Wet Mount andHanging DropExercise 43 Flagella StainExercise 44 Streak-Plate IsolationExercise 45 Loop DilutionExercise 46 Spread-PlateExercise 47 Fluid Thioglycollate MediumExercise 48 Mannitol Salt AgarExercise 49 MacConkey AgarExercise 50 Desoxycholate AgarExercise 51 Endo AgarExercise 52 Eosin Methylene Blue AgarExercise 53 Hektoen Enteric AgarExercise 54 Xylose Lysine Desoxycholate AgarExercise 55 Blood AgarExercise 56 Motility MediumExercise 57 SIM MediumExercise 58 Kligler's Iron AgarExercise 59 Triple Sugar Iron AgarExercise 60 Lysine Iron AgarExercise 61 Litmus MilkExercise 62 Oxidation-Fermentation TestExercise 63 Phenol Red BrothExercise 64 Purple BrothExercise 65 Methyl Red and Voges-ProskauerTestsExercise 66 Catalase TestExercise 67 Oxidase TestExercise 68 Nitrate Reduction TestExercise 69 Coagulase TestExercise 70 Citrate TestExercise 71 Malonate TestExercise 72 Decarboxylation TestExercise 73 Phenylalanine Deaminase TestExercise 74 Bile Esculin TestExercise 75 Starch HydrolysisExercise 76 ONPG TestExercise 77 Urease TestExercise 78 Casease TestExercise 79 Gelatinase TestExercise 80 DNase TestExercise 81 Lipase TestExercise 82 CAMP TestExercise 83 PYR TestExercise 84 API 20E SystemExercise 85 Enterotube II SystemExercise 86 Antibiotic Disk Sensitivity Tests Exercise 87 B-Lactamase TestExercise 88 Viable Plate CountExercise 89 Direct Cell CountAppendix A: Spectrophotometric Determination of BacterialGrowth: Use of the SpectrophotometerAppendix B: Use of Pipettes in the LaboratoryAppendix C: Preparation of Culture MediaAppendix D: Media, Reagents, and Stain FormulasAppendix E: Data Sheet for Unknown Identification