Laboratory Applications in Microbiology: A Case Study Approach has been designed to help create accomplished microbiologists. Case studies illustrate the applicability of skills in the microbiology lab, while still holding the attention of every student who has ever said “do we have to know this?” Each exercise has been structured from the bottom up, scaffolding knowledge and relying on metacognition to ensure students understand the goals of an exercise, anticipate errors, acquire the skills needed for success, and eventually master the topic at hand.;/div>

Table of Contents:
1 Safety Considerations in the Microbiology Laboratory 2 Microscopy and Measurement of Microscopic Specimens 3 A Survey of Protists 4 A Survey of Fungi 5 A Survey of Parasitic Worms 6 Ubiquity of Microorganisms 7 Aseptic and Pure Culture Techniques 8 Simple Staining, Negative Staining, and Gram Staining 9 Capsular Staining 10 Endospore Staining 11 Acid-Fast Staining 12 Viable Plate Count 13 Cultivation of Anaerobes 14 Temperature Effects on Bacterial Growth and Survival 15 pH and Microbial Growth 16 Effects of Osmotic Pressure on Bacterial Growth 17 Lethal Effects of Ultraviolet Light 18 Evaluation of Disinfectants 19 Effectiveness of Hand Scrubbing 20 Antimicrobic Sensitivity Testing: Kirby-Bauer, Tube Dilution, and ETEST(C) Methods 21 Simulated Epidemic 22 Morbidity and Mortality Weekly Report 23 Bacterial Transformation 24 The Ames Test 25 DNA Extraction from Bacterial Cells 26 DNA Profiling 27 Blood Typing 28 Rapid Identification of Staphylococcus aureus Using Latex Agglutination Testing 29 Slide Agglutination 30 Enzyme-Linked Immunosorbent Assay (ELISA) 31 Biofilm Culture and Examination 32 Measures of Water Quality: Most Probable Number Procedure 33 Measures of Water Quality: Membrane Filtration Method 34 Measures of Milk Quality: Methylene Blue Reductase Test 35 Bacterial Counts of Food 36 Isolation and Identification of Staphylococci 37 Isolation and Identification of Streptococci 38 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacterlaceae 39 Differential White Blood Cell Count 40 Identification of Bacterial Unknowns 41 Colony Morphology 42 Growth in Solid and Liquid Media 43 Motilty Methods: Wet Mount and Hanging Drop 44 Flagella Stain 45 Streak-Plate Isolation 46 Loop Dilution 47 Spread-Plate 48 Fluid Thioglycollate Medium 49 CHROMagar Orientation Medium 50 Mannitol Salt Agar 51 MacConkey Agar 52 Desoxycholate Agar 53 Endo Agar 54 Eosin Methylene Blue Agar 55 Hektoen Enteric Agar 56 Xylose Lysine Desoxycholate Agar 57 Blood Agar 58 Motility Medium 59 SIM Medium 60 Kligler's Iron Agar 61 Triple Sugar Iron Agar 62 Lysine Iron Agar 63 Litmus Milk 64 Oxidation-Fermentation Test 65 Phenol Red Carbohydrate Broth 66 Purple Carbohydrate Broth 67 Methyl Red and Voges-ProskauerTests 68 Catalase Test 69 Oxidase Test 70 Nitrate Reduction Test 71 Coagulase Test 72 Citrate Test 73 Malonate Test 74 Amino Acid Decarboxylation Test 75 Phenylalanine Deaminase Test 76 Bile Esculin Test 77 Starch Hydrolysis 78 ONPG Test 79 Urease Test 80 Casease Test 81 Gelatinase Test 82 DNase Test 83 Lipase Test 84 CAMP Test 85 PYR Test 86 API 20E System 87 EnteroPluri-Test System 88 Antibiotic Disk Sensitivity Tests 89 B-Lactamase Test 90 Viable Plate Count 91 Direct Cell Count