The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Table of Contents:
Vectors for Expressing Cloned Genes:G. Sczakiel and K. Maeda, Vector pPLEX for Expression of Nonfusion Polypeptides in Escherichia coli.H. Orum and L.K. Poulsen, In-Frame Gene Fusion.S. Udaka and H. Yamagata, High Level Secretion of Heterologous Proteins by Bacillus brevis.S.E. Kane and M.M. Gottesman, Use of Multidrug Resistance Gene in Mammalian Expression Vectors.A. Lieber, V. Sandig, W. Sommer, S. B~adahring, and M. Strauss, Stable High Level Gene Expression in Mammalian Cells by T7 Phage RNA Polymerase.R. T~adopfer, C. Maas, C. H~adoricke-Grandpierre, J. Schell, and H.-H. Steinbiss, Expression Vectors for High Level Gene Expression in Dicotyledonous and Monocotyledonous Plants.K.D. MacFerrin, L. Chen, M.P. Terranova, S.L. Schreiber, and G.L. Verdine, Overproduction of Proteins Using the Expression-Cassette Polymerase Chain Reaction.T.L. Pauls and M.W. Berchtold, Efficient cDNA Amplification and Expression Using PCR Technology.V.I. Baranov and A.S. Spirin, Gene Expression in a Cell-Free System on the Preparative Scale.B. Dasmahapatra, Cell-Free Expression Vector: Use of Insect Virus Translational Initiation Signal for in Vitro Gene Expression.C. Coleclough, Cell-Free Expression of Large Collections of cDNA Clones Using Positive Selection, ~gl Phage Vectors.Mutagenesis and Gene Disruption:B. Hofer and B. K~aduhlein, Site-Specific Mutagenesis in Plasmids: A Gapped Circle Method.D.B. Olsen, J.R. Sayers, and F. Eckstein, Site-Directed Mutagenesis of Single-Stranded and Double-Stranded DNA by the Phosphorothioate Approach.L.-J. Zhao, Q.X. Zhang, and R. Padmanabhan, PCR-Based Point Mutagenesis Protocol.G.P. Smith and J.K. Scott, Libraries of Peptides and Proteins Displayed on Filamentous Phage.M.M.Y. Waye, Use of M13 Ping-Pong Vectors and T4DNA Polymerase in Oligodeoxynucleotide-Directed Mutagenesis.R.M. Horton, S.N. Ho, J.K. Pullen, H.D. Hunt, Z. Cai, and L.R. Pease, Gene Splicing by Overlap Extension.C. Batt, Y. Cho, and A.C. Jamieson, Selection of Oligodeoxynucleotide-Directed Mutants.J.A. Saleeba and R.G.H. Cotton, Chemical Cleavage of Mismatch to Detect Mutations.D.C. Kaslow and D.J. Rawlings, Restriction Sites into Double-Stranded Plasmid DNA.T.C. Huffaker, Expression and Screening in Yeast of Genes Mutagenized in Vitro.W.S. Reznikoff, R. Jilk, M.P. Krebs, J.C. Makris, P.L. Nordmann, M. Weinreich, and T. Wiegand, Tn5 lacZ Translation Fusion Element: Isolation and Analysis of Tranposition Mutants.Screening Libraries, Identifying and Mapping Genes:J. Chao, K.X. Chai, and L. Chao, Screening of cDNA Library Using Radiolabeled Antigen.P. Karlovsky, Alternatives to X-Galactopyranoside in Screening Recombinant Clones Based on pUC-Derived Plasmid Vectors.G.V. Paddock, Rapid Colony Hybridization on Paper Using Oligonucleotide Probes.A. Poustka, Large Insert Linking-Clone Libraries: Construction and Use.A. Poustka, Construction and Use of Chromosome Jumping Libraries.C.Y. Yu, L.-c. Wu, L. Buluwela, and C. Milstein, Cosmid Cloning and Walking to Map Human CD1 Leukocyte Differentiation AntigenGenes:I. Bronstein, J.C. Voyta, O.J. Murphy, R. Tizard, C.W. Ehrenfels, and R.L. Cate, Detection of DNA in Southern Blots with Chemiluminescence.H.S. Rye, S. Yue, M.A. Quesada, R.P. Haugland, R.A. Mathies, and A.N. Glazer, Picogram Detection of Stable Dye-DNA Intercalation Complexes with a Two-Color Laser-Excited Confocal Fluoresence Gel Scanner.C. Cantrell and E. Lai, Alginate as Matrix Support for Yeast Artificial Chromosome Cloning.V. Shyamala and G.F.-L. Ames, Genome Walking by Single Specific Primer-Polymerase Chain Reaction.M.C.-T. Hu and N. Davidson, Mapping Transcription Start Points with T4 DNA Polymerase.Methods for Transforming Animal and Plant Cells:H. Potter, Application of Electroporation in Recombinant DNA Technology.J.R. Simon, Transformation of Intact Yeast Cells by Electroporation.J.C. Sanford, F.D. Smith, and J.A. Russell, Optimizing Biolistic Process for Biological Applications.J.E. Boynton and N. Gillham, Chloroplast Transformation in Chlamydomonas.H. Daniell, Foreign Gene Expression in Chloroplasts on Higher Plants Mediated by Tungsten Particle Bombardment.G.P. Mazzara, Generation and Analysis of Vaccinia Virus Recombinants.A.D. Miller, D.G. Miller, J.V. Garcia, and C.M. Lynch, Use of Retroviral Vectors for Gene Transfer amd Expression.J.-P. Loeffler and J.-P. Behr, Gene Transfer into Primary and Established Mammalian Cell Lines with Lipopolyamine-Coated DNA.M. Cotten, E. Wagner, and M.L. Birnstiel, Receptor-Mediated Transport of DNA into Eukaryotic Cells.V.J. Dwarki, R.W. Malone, and I.M. Verma, Cationic Liposome-Mediated RNA Transfection.Author Index.Subject Index.